{{Short description|Protein-coding gene in the species Homo sapiens}} {{Infobox_gene}} '''Methyl-CpG-binding domain protein 2''' is a protein that in humans is encoded by the ''MBD2'' gene.<ref name="pmid9774669">{{cite journal | vauthors = Hendrich B, Bird A | title = Identification and Characterization of a Family of Mammalian Methyl-CpG Binding Proteins | journal = Mol Cell Biol | volume = 18 | issue = 11 | pages = 6538–47 | date = November 1998 | pmid = 9774669 | pmc = 109239 | doi = 10.1128/mcb.18.11.6538}}</ref><ref name="pmid10441743">{{cite journal | vauthors = Hendrich B, Abbott C, McQueen H, Chambers D, Cross S, Bird A | title = Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes | journal = Mamm Genome | volume = 10 | issue = 9 | pages = 906–12 | date = September 1999 | pmid = 10441743 | doi = 10.1007/s003359901112 | s2cid = 819148 }}</ref>
== Function ==
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1, and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by these genes may function as a mediator of the biological consequences of the methylation signal. It is also reported that MBD2 and MBD3 recruit the NuRD complex to regions of DNA depending on their selective binding of methylated CpG sites. Therefore, MBD2/NuRD and MBD3/NuRD define two distinct protein complexes with different biochemical and functional properties.<ref name="Guezennec">{{cite journal |title=MBD2/NuRD and MBD3/NuRD, Two Distinct Complexes with Different Biochemical and Functional Properties |journal=Molecular and Cellular Biology |year=2006 |last=Guezennec |first=Xavier |display-authors=et al. |volume=26 |issue=3 |pages=843–851 |pmid=16428440 |pmc=1347035 |doi=10.1128/MCB.26.3.843-851.2006 }}</ref>
== Interactions ==
Methyl-CpG-binding domain protein 2 has been shown to interact with: {{div col|colwidth=20em}} * GATAD2B,<ref name = pmid12183469/><ref name = pmid11756549>{{cite journal | vauthors = Feng Q, Cao R, Xia L, Erdjument-Bromage H, Tempst P, Zhang Y | title = Identification and functional characterization of the p66/p68 components of the MeCP1 complex | journal = Mol. Cell. Biol. | volume = 22 | issue = 2 | pages = 536–46 | date = January 2002 | pmid = 11756549 | pmc = 139742 | doi = 10.1128/mcb.22.2.536-546.2002}}</ref> * HDAC1,<ref name = pmid12183469>{{cite journal | vauthors = Brackertz M, Boeke J, Zhang R, Renkawitz R | title = Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3 | journal = J. Biol. Chem. | volume = 277 | issue = 43 | pages = 40958–66 | date = October 2002 | pmid = 12183469 | doi = 10.1074/jbc.M207467200 | doi-access = free }}</ref><ref name = pmid10471499>{{cite journal | vauthors = Ng HH, Zhang Y, Hendrich B, Johnson CA, Turner BM, Erdjument-Bromage H, Tempst P, Reinberg D, Bird A | title = MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex | journal = Nat. Genet. | volume = 23 | issue = 1 | pages = 58–61 | date = September 1999 | pmid = 10471499 | doi = 10.1038/12659 | hdl = 1842/684 | s2cid = 6147725 | hdl-access = free }}</ref><ref name = pmid10444591>{{cite journal | vauthors = Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D | title = Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation | journal = Genes Dev. | volume = 13 | issue = 15 | pages = 1924–35 | date = August 1999 | pmid = 10444591 | pmc = 316920 | doi = 10.1101/gad.13.15.1924}}</ref> * Histone deacetylase 2,<ref name = pmid12183469/><ref name = pmid10471499/><ref name = pmid10444591/> * MBD3<ref name = pmid10444591/><ref name = pmid15456747>{{cite journal | vauthors = Jiang CL, Jin SG, Pfeifer GP | title = MBD3L1 is a transcriptional repressor that interacts with methyl-CpG-binding protein 2 (MBD2) and components of the NuRD complex | journal = J. Biol. Chem. | volume = 279 | issue = 50 | pages = 52456–64 | date = December 2004 | pmid = 15456747 | doi = 10.1074/jbc.M409149200 | doi-access = free }}</ref> * MIZF,<ref name = pmid11553631>{{cite journal | vauthors = Sekimata M, Takahashi A, Murakami-Sekimata A, Homma Y | title = Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2 | journal = J. Biol. Chem. | volume = 276 | issue = 46 | pages = 42632–8 | date = November 2001 | pmid = 11553631 | doi = 10.1074/jbc.M107048200 | doi-access = free }}</ref> and * SIN3A.<ref name = pmid10950960>{{cite journal | vauthors = Boeke J, Ammerpohl O, Kegel S, Moehren U, Renkawitz R | title = The minimal repression domain of MBD2b overlaps with the methyl-CpG-binding domain and binds directly to Sin3A | journal = J. Biol. Chem. | volume = 275 | issue = 45 | pages = 34963–7 | date = November 2000 | pmid = 10950960 | doi = 10.1074/jbc.M005929200 | doi-access = free }}</ref> {{Div col end}}
== References == {{reflist}}
== Further reading == {{refbegin|35em}} * {{cite journal | vauthors = Scanlan MJ, Chen YT, Williamson B, Gure AO, Stockert E, Gordan JD, Türeci O, Sahin U, Pfreundschuh M, Old LJ | title = Characterization of human colon cancer antigens recognized by autologous antibodies | journal = Int. J. Cancer | volume = 76 | issue = 5 | pages = 652–8 | year = 1998 | pmid = 9610721 | doi = 10.1002/(SICI)1097-0215(19980529)76:5<652::AID-IJC7>3.0.CO;2-P | doi-access = free }} * {{cite journal | vauthors = Zhang Y, LeRoy G, Seelig HP, Lane WS, Reinberg D | title = The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities | journal = Cell | volume = 95 | issue = 2 | pages = 279–89 | year = 1998 | pmid = 9790534 | doi = 10.1016/S0092-8674(00)81758-4 | s2cid = 18786866 | doi-access = free }} * {{cite journal | vauthors = Tong JK, Hassig CA, Schnitzler GR, Kingston RE, Schreiber SL | title = Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex | journal = Nature | volume = 395 | issue = 6705 | pages = 917–21 | year = 1998 | pmid = 9804427 | doi = 10.1038/27699 | bibcode = 1998Natur.395..917T | s2cid = 4355885 }} * {{cite journal | vauthors = Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M | title = A mammalian protein with specific demethylase activity for mCpG DNA | journal = Nature | volume = 397 | issue = 6720 | pages = 579–83 | year = 1999 | pmid = 10050851 | doi = 10.1038/17533 | bibcode = 1999Natur.397..579B | s2cid = 4408031 }} * {{cite journal | vauthors = Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A, Reinberg D | title = Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation | journal = Genes Dev. | volume = 13 | issue = 15 | pages = 1924–35 | year = 1999 | pmid = 10444591 | pmc = 316920 | doi = 10.1101/gad.13.15.1924 }} * {{cite journal | vauthors = Ng HH, Zhang Y, Hendrich B, Johnson CA, Turner BM, Erdjument-Bromage H, Tempst P, Reinberg D, Bird A | title = MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex | journal = Nat. Genet. | volume = 23 | issue = 1 | pages = 58–61 | year = 1999 | pmid = 10471499 | doi = 10.1038/12659 | hdl = 1842/684 | s2cid = 6147725 | hdl-access = free }} * {{cite journal | vauthors = Tatematsu KI, Yamazaki T, Ishikawa F | title = MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase | journal = Genes Cells | volume = 5 | issue = 8 | pages = 677–88 | year = 2000 | pmid = 10947852 | doi = 10.1046/j.1365-2443.2000.00359.x | s2cid = 25185979 | doi-access = free }} * {{cite journal | vauthors = Boeke J, Ammerpohl O, Kegel S, Moehren U, Renkawitz R | title = The minimal repression domain of MBD2b overlaps with the methyl-CpG-binding domain and binds directly to Sin3A | journal = J. Biol. Chem. | volume = 275 | issue = 45 | pages = 34963–7 | year = 2001 | pmid = 10950960 | doi = 10.1074/jbc.M005929200 | doi-access = free }} * {{cite journal | vauthors = Humphrey GW, Wang Y, Russanova VR, Hirai T, Qin J, Nakatani Y, Howard BH | title = Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1 | journal = J. Biol. Chem. | volume = 276 | issue = 9 | pages = 6817–24 | year = 2001 | pmid = 11102443 | doi = 10.1074/jbc.M007372200 | doi-access = free }} * {{cite journal | vauthors = Feng Q, Zhang Y | title = The MeCP1 complex represses transcription through preferential binding, remodeling, and deacetylating methylated nucleosomes | journal = Genes Dev. | volume = 15 | issue = 7 | pages = 827–32 | year = 2001 | pmid = 11297506 | pmc = 312663 | doi = 10.1101/gad.876201 }} * {{cite journal | vauthors = Sekimata M, Takahashi A, Murakami-Sekimata A, Homma Y | title = Involvement of a novel zinc finger protein, MIZF, in transcriptional repression by interacting with a methyl-CpG-binding protein, MBD2 | journal = J. Biol. Chem. | volume = 276 | issue = 46 | pages = 42632–8 | year = 2001 | pmid = 11553631 | doi = 10.1074/jbc.M107048200 | doi-access = free }} * {{cite journal | vauthors = Müller-Tidow C, Kügler K, Diederichs S, Klümpen S, Möller M, Vogt U, Metzger R, Schneider PM, Berdel WE, Serve H | title = Loss of expression of HDAC-recruiting methyl-CpG-binding domain proteins in human cancer | journal = Br. J. Cancer | volume = 85 | issue = 8 | pages = 1168–74 | year = 2001 | pmid = 11710831 | pmc = 2375156 | doi = 10.1054/bjoc.2001.2041 }} * {{cite journal | vauthors = Feng Q, Cao R, Xia L, Erdjument-Bromage H, Tempst P, Zhang Y | title = Identification and Functional Characterization of the p66/p68 Components of the MeCP1 Complex | journal = Mol. Cell. Biol. | volume = 22 | issue = 2 | pages = 536–46 | year = 2002 | pmid = 11756549 | pmc = 139742 | doi = 10.1128/MCB.22.2.536-546.2002 }} * {{cite journal | vauthors = Bakker J, Lin X, Nelson WG | title = Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells | journal = J. Biol. Chem. | volume = 277 | issue = 25 | pages = 22573–80 | year = 2002 | pmid = 11960994 | doi = 10.1074/jbc.M203009200 | doi-access = free }} * {{cite journal | vauthors = Saito M, Ishikawa F | title = The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2 | journal = J. Biol. Chem. | volume = 277 | issue = 38 | pages = 35434–9 | year = 2002 | pmid = 12124384 | doi = 10.1074/jbc.M203455200 | doi-access = free }} * {{cite journal | vauthors = Detich N, Theberge J, Szyf M | title = Promoter-specific activation and demethylation by MBD2/demethylase | journal = J. Biol. Chem. | volume = 277 | issue = 39 | pages = 35791–4 | year = 2002 | pmid = 12177048 | doi = 10.1074/jbc.C200408200 | doi-access = free }} * {{cite journal | vauthors = Brackertz M, Boeke J, Zhang R, Renkawitz R | title = Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3 | journal = J. Biol. Chem. | volume = 277 | issue = 43 | pages = 40958–66 | year = 2002 | pmid = 12183469 | doi = 10.1074/jbc.M207467200 | doi-access = free }} * {{cite journal | vauthors = Krithivas A, Fujimuro M, Weidner M, Young DB, Hayward SD | title = Protein Interactions Targeting the Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus to Cell Chromosomes | journal = J. Virol. | volume = 76 | issue = 22 | pages = 11596–604 | year = 2002 | pmid = 12388720 | pmc = 136775 | doi = 10.1128/JVI.76.22.11596-11604.2002 }} {{refend}}
Category:Human proteins