{{multiple issues| {{technical|date=October 2017}} {{no footnotes|date=October 2017}} {{missing information|Extravidin and NeutraLite brand names|date=November 2020}} }} '''Neutralite Avidin''' protein is a deglycosylated version of chicken avidin, with a mass of approximately 60,000 daltons. As a result of carbohydrate removal, lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is not necessary for this activity. Avidin has a high pI but NeutrAvidin has a near-neutral pI (pH 6.3), minimizing non-specific interactions with the negatively-charged cell surface or with DNA/RNA. Neutravidin still has lysine residues that remain available for derivatization or conjugation.
Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin (''K''<sub>d</sub> = 10<sup>−15 </sup>M). In biochemical applications, streptavidin, which also binds very tightly to biotin, may be used interchangeably with NeutrAvidin.
Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane-impermeable biotin reagent, then specifically captured using a NeutrAvidin support.
== References == * Bayer, Ed: "[https://www.weizmann.ac.il/Biomolecular_Sciences/Bayer/research-activities/avidin-biotin-system The avidin-biotin system]", Dept. of Biological Chemistry, Weizmann Institute of Science, Israel * {{citation |author=Y. Hiller, J.M. Gershoni, E. A. Bayer, M. Wilchek |date=1987 |doi=10.1042/bj2480167 |issue=1 |pages=167–171 |periodical=Biochemical Journal |pmc=1148514 |pmid=3435435 |title=Biotin binding to avidin. Oligosaccharide side chain not required for ligand association |volume=248}}<!-- auto-translated from German by Module:CS1 translator --> * {{citation |author=Edward A. Bayer, Fabien De Meester, Tikva Kulik, Meir Wilchek |date=1995 |doi=10.1007/BF02783477 |issue=1 |pages=1–9 |periodical=Applied Biochemistry and Biotechnology |title=Preparation of deglycosylated egg white avidin |volume=53|pmid=7625822 }}<!-- auto-translated from German by Module:CS1 translator --> * {{citation |author=Ari T. Marttila, Olli H. Laitinen, Kari J. Airenne, Tikva Kulik, Edward A. Bayer, Meir Wilchek, Markku S. Kulomaa |date=2000 |doi=10.1016/S0014-5793(00)01119-4 |issue=1 |pages=31–36 |periodical=FEBS Letters |title=Recombinant NeutraLite Avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties |volume=467|pmid=10664451 |bibcode=2000FEBSL.467...31M }}<!-- auto-translated from German by Module:CS1 translator -->
Category:Proteins
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