Histology

{{short description|Study of the microscopic anatomy of cells and tissues of plants and animals}} {{redirect|Histography|the study of history as a science|Historiography}} [[File:Slide_under_a_microscope.jpg|thumb|Histologic specimen being placed on the stage of an optical microscope]] [[File:Emphysema H and E.jpg|right|300px|thumb|Human lung tissue stained with hematoxylin and eosin as seen under a microscope]]

'''Histology''',{{refn|name=help|group= help |The word ''histology'' ({{IPAc-en|h|ɪ|s|ˈ|t|ɒ|l|ə|dʒ|i}}) is Neo-Latin, combining ''histo-'' + ''-logy'' to mean "study of tissue"; from the Greek words {{wikt-lang|grc|ἱστός}} ({{grc-transl|ἱστός}}) "tissue", and {{wikt-lang|grc|-λογία}} ({{grc-transl|-λογία}}) "study of". }} also known as '''microscopic anatomy''', '''microanatomy'''<ref name="Collins" /> or '''histoanatomy''',<ref>{{cite book |author1=Kiminori Sato |title=Functional Histoanatomy of the Human Larynx |date=2018 |publisher=Springer |isbn=9789811055867 |url=https://books.google.com/books?id=fZRIDwAAQBAJ&pg=PR3}}</ref><ref>{{cite book |author1=Hamid M. Said |title=Physiology of the Gastrointestinal Tract |date=2012 |publisher=Academic Press |isbn=9780123820273 |page=630 |edition=5 |url=https://books.google.com/books?id=ytWBLAy1FGQC&pg=PA630}}</ref> is the branch of biology that studies the microscopic anatomy of biological tissues.<ref name="Britannica histology" /><ref name="Defined terms histology" /><ref name="Maximow and Bloom, 1957" /><ref name="Leeson and Leeson, 1981" /> Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope.<ref name="Leeson and Leeson, 1981" /><ref name="Stedman's medical dictionary" />

Historically, microscopic anatomy was divided into organology, the study of organs, histology, the study of tissues, and cytology, the study of cells, although modern usage places all of these topics under the field of histology.<ref name="Leeson and Leeson, 1981" /> In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue.<ref name="Leeson and Leeson, 1981" /><ref name="Stedman's medical dictionary" /> In the field of paleontology, the term paleohistology refers to the histology of fossil organisms.<ref name="Padian and Lamm, 2013" /><ref name="Canoville and Chinsamy, 2015" /> {{TOC limit}}

==Biological tissues==

===Animal tissue classification === {{main|Anatomy#Animal tissues}}

There are four basic types of animal tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue.<ref name="Leeson and Leeson, 1981" /><ref name="Ross and Pawlina, 2016" /> All animal tissues are considered to be subtypes of these four principal tissue types (for example, blood is classified as connective tissue, since the blood cells are suspended in an extracellular matrix, the plasma).<ref name="Ross and Pawlina, 2016" /> {{columns-list|colwidth=20em| * '''Epithelium''' ** Simple epithelium. *** Simple squamous epithelium. *** Simple cuboidal epithelium. *** Simple columnar epithelium. ** Pseudostratified columnar epithelium ** Stratified epithelium *** Stratified squamous epithelium *** Stratified cuboidal epithelium *** Stratified columnar epithelium *** Transitional epithelium ** Multicellular glands * '''Muscle tissue''' ** Smooth muscle ** Skeletal muscle ** Cardiac muscle * '''Connective tissue''' ** General connective tissue *** Loose connective tissue *** Dense connective tissue ** Special connective tissue *** Cartilage *** Bone *** Hemopoietic *** Blood *** Lymph *'''Nervous tissue''' ** Central nervous system ** Peripheral nervous system ** Special receptors }} .

===Plant tissue classification=== [[File:Alliaria petiolata, stalk, cross section, Etzold green.jpg|thumb|right|300px|Histologic section of a plant stem (''Alliaria petiolata'')]] {{main|Plant Anatomy}}

For plants, the study of their tissues falls under the field of plant anatomy, with the following four main types: * Dermal tissue * Vascular tissue * Ground tissue * Meristematic tissue

==Medical histology==

Histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue.<ref name="Leeson and Leeson, 1981" /><ref name="Stedman's medical dictionary" /> It is an important part of anatomical pathology and surgical pathology, as accurate diagnosis of cancer and other diseases often requires histopathological examination of tissue samples.<ref name="Rosai, 2007" /> Trained physicians, frequently licensed pathologists, perform histopathological examination and provide diagnostic information based on their observations.

===Occupations=== The field of histology that includes the preparation of tissues for microscopic examination is known as histotechnology. Job titles for the trained personnel who prepare histological specimens for examination are numerous and include histotechnicians, histotechnologists,<ref name="Titford and Bowman, 2012" /> histology technicians and technologists, medical laboratory technicians, and biomedical scientists.

==Sample preparation==

Most histological samples need preparation before microscopic observation; these methods depend on the specimen and method of observation.<ref name="Ross and Pawlina, 2016" />

===Fixation === {{main|Fixation (histology)}}

[[File:Stigmatella personata thin section.jpg|thumb|300px|right|Histologic section of a fossilized invertebrate. Ordovician bryozoan.]]

Chemical fixatives are used to preserve and maintain the structure of tissues and cells; fixation also hardens tissues which aids in cutting the thin sections of tissue needed for observation under the microscope.<ref name="Leeson and Leeson, 1981" /><ref name="Bancroft and Stevens, 1982" /> Fixatives generally preserve tissues (and cells) by irreversibly cross-linking proteins.<ref name="Bancroft and Stevens, 1982" /> The most widely used fixative for light microscopy is 10% neutral buffered formalin, or NBF (4% formaldehyde in phosphate buffered saline).<ref name="Wick, 2019" /><ref name="Bancroft and Stevens, 1982" /><ref name="Ross and Pawlina, 2016" />

For electron microscopy, the most commonly used fixative is glutaraldehyde, usually as a 2.5% solution in phosphate buffered saline.<ref name="Ross and Pawlina, 2016" /> Other fixatives used for electron microscopy are osmium tetroxide or uranyl acetate.<ref name="Ross and Pawlina, 2016" />

The main action of these aldehyde fixatives is to cross-link amino groups in proteins through the formation of methylene bridges ({{chem2|\sCH2\s}}), in the case of formaldehyde, or by C₅H₁₀ cross-links in the case of glutaraldehyde. This process, while preserving the structural integrity of the cells and tissue can damage the biological functionality of proteins, particularly enzymes.

Formalin fixation leads to degradation of mRNA, miRNA, and DNA as well as denaturation and modification of proteins in tissues. However, extraction and analysis of nucleic acids and proteins from formalin-fixed, paraffin-embedded tissues is possible using appropriate protocols.<ref name="Weiss et al., 2011" /><ref name="Bennike et al., 2016" />

===Selection and trimming=== thumb|Items used for submitting specimens: (Biopsy) wrap, (biopsy) sponge, (tissue processing) cassette and (biopsy) bag. ''Selection'' is the choice of relevant tissue in cases where it is not necessary to put the entire original tissue mass through further processing. The remainder may remain fixed in case it needs to be examined at a later time.

''Trimming'' is the cutting of tissue samples in order to expose the relevant surfaces for later sectioning. It also creates tissue samples of appropriate size to fit into cassettes.<ref name="SlaouiFiette2011">{{cite book|last1=Slaoui|first1=Mohamed|title=Drug Safety Evaluation|last2=Fiette|first2=Laurence|chapter=Histopathology Procedures: From Tissue Sampling to Histopathological Evaluation|volume=691|year=2011|pages=69–82|issn=1064-3745|doi=10.1007/978-1-60761-849-2_4|pmid=20972747|series=Methods in Molecular Biology|isbn=978-1-60327-186-8}}</ref>

===Embedding=== Tissues are embedded in a harder medium both as a support and to allow the cutting of thin tissue slices.<ref name="Ross and Pawlina, 2016" /><ref name="Leeson and Leeson, 1981" /> In general, water must first be removed from tissues (dehydration) and replaced with a medium that either solidifies directly, or with an intermediary fluid (clearing) that is miscible with the embedding media.<ref name="Bancroft and Stevens, 1982" />

==== Paraffin wax ==== thumb|left|Histologic sample being embedded in paraffin wax (Tissue is held at the bottom of a metal mold, and more molten paraffin is poured over it to fill it.)

For light microscopy, paraffin wax is the most frequently used embedding material.<ref name="Bancroft and Stevens, 1982" /><ref name="Wick, 2019" /> Paraffin is immiscible with water, the main constituent of biological tissue, so it must first be removed in a series of dehydration steps.<ref name="Bancroft and Stevens, 1982" /> Samples are transferred through a series of progressively more concentrated ethanol baths, up to 100% ethanol to remove remaining traces of water.<ref name="Ross and Pawlina, 2016" /><ref name="Bancroft and Stevens, 1982" /> Dehydration is followed by a ''clearing agent'' (typically xylene<ref name="Wick, 2019" /> although other environmental safe substitutes are in use<ref name="Wick, 2019" />) which removes the alcohol and is miscible with the wax, finally melted paraffin wax is added to replace the xylene and infiltrate the tissue.<ref name="Ross and Pawlina, 2016" /> In most histology, or histopathology laboratories the dehydration, clearing, and wax infiltration are carried out in ''tissue processors'' which automate this process.<ref name="Wick, 2019" /> Once infiltrated in paraffin, tissues are oriented in molds which are filled with wax; once positioned, the wax is cooled, solidifying the block and tissue.<ref name="Wick, 2019" /><ref name="Bancroft and Stevens, 1982" />

==== Other materials ====

Paraffin wax does not always provide a sufficiently hard matrix for cutting very thin sections (which are especially important for electron microscopy).<ref name="Bancroft and Stevens, 1982" /> Paraffin wax may also be too soft in relation to the tissue, the heat of the melted wax may alter the tissue in undesirable ways, or the dehydrating or clearing chemicals may harm the tissue.<ref name="Bancroft and Stevens, 1982" /> Alternatives to paraffin wax include, epoxy, acrylic, agar, gelatin, celloidin, and other types of waxes.<ref name="Bancroft and Stevens, 1982" /><ref name="Drury and Wallington, 1980" />

In electron microscopy epoxy resins are the most commonly employed embedding media,<ref name="Ross and Pawlina, 2016" /> but acrylic resins are also used, particularly where immunohistochemistry is required.

For tissues to be cut in a frozen state, tissues are placed in a water-based embedding medium. Pre-frozen tissues are placed into molds with the liquid embedding material, usually a water-based glycol, OCT, TBS, Cryogen, or resin, which is then frozen to form hardened blocks.

===Sectioning=== {{Main|Microtome}}

thumb|Histologic sample being cut on a microtome

For light microscopy, a knife mounted in a microtome is used to cut tissue sections (typically between 5-15 micrometers thick) which are mounted on a glass microscope slide.<ref name="Ross and Pawlina, 2016" /> For transmission electron microscopy (TEM), a diamond or glass knife mounted in an ultramicrotome is used to cut between 50 and 150 nanometer thick tissue sections.<ref name="Ross and Pawlina, 2016" />

A limited number of manufacturers are recognized for their production of microtomes, including vibrating microtomes commonly referred to as vibratomes, primarily for research and clinical studies. Additionally, Leica Biosystems is known for its production of products related to light microscopy in the context of research and clinical studies.<ref>{{cite web | url=https://www2.leicabiosystems.com/in/microtomes-JUN20/ | title=Rotary Microtomes - Leica Biosystems }}</ref>

===Staining=== {{Main|Staining}}

Biological tissue has little inherent contrast in either the light or electron microscope.<ref name="Drury and Wallington, 1980" /> Staining is employed to give both contrast to the tissue as well as highlighting particular features of interest. When the stain is used to target a specific chemical component of the tissue (and not the general structure), the term histochemistry is used.<ref name="Ross and Pawlina, 2016" />

====Light microscopy==== [[Image:Masson's trichrome staining on rat's trachea.jpg|thumb|300px|left|Masson's trichrome staining on rat trachea ]] Hematoxylin and eosin (H&E stain) is one of the most commonly used stains in histology to show the general structure of the tissue.<ref name="Ross and Pawlina, 2016" /><ref name="Dapson and Horobin, 2009" /> Hematoxylin stains cell nuclei blue; eosin, an acidic dye, stains the cytoplasm and other tissues in different stains of pink.<ref name="Ross and Pawlina, 2016" /><ref name="Bancroft and Stevens, 1982" />

In contrast to H&E, which is used as a general stain, there are many techniques that more selectively stain cells, cellular components, and specific substances.<ref name="Bancroft and Stevens, 1982" /> A commonly performed histochemical technique that targets a specific chemical is the Perls' Prussian blue reaction, used to demonstrate iron deposits<ref name="Bancroft and Stevens, 1982" /> in diseases like hemochromatosis. The Nissl method for Nissl substance and Golgi's method (and related silver stains) are useful in identifying neurons are other examples of more specific stains.<ref name="Bancroft and Stevens, 1982" />

====Historadiography====

In historadiography, a slide (sometimes stained histochemically) is X-rayed. More commonly, autoradiography is used in visualizing the locations to which a radioactive substance has been transported within the body, such as cells in S phase (undergoing DNA replication) which incorporate tritiated thymidine, or sites to which radiolabeled nucleic acid probes bind in in situ hybridization. For autoradiography on a microscopic level, the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film. Individual silver grains in the film are visualized with dark field microscopy.

====Immunohistochemistry==== {{main|immunohistochemistry}}

Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids. This process is called immunohistochemistry, or when the stain is a fluorescent molecule, immunofluorescence. This technique has greatly increased the ability to identify categories of cells under a microscope. Other advanced techniques, such as nonradioactive ''in situ'' hybridization, can be combined with immunochemistry to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification (especially alkaline phosphatase and tyramide signal amplification). Fluorescence microscopy and confocal microscopy are used to detect fluorescent signals with good intracellular detail.

====Electron microscopy==== {{Main|Electron microscope#Sample preparation}}

For electron microscopy heavy metals are typically used to stain tissue sections.<ref name="Ross and Pawlina, 2016" /> Uranyl acetate and lead citrate are commonly used to impart contrast to tissue in the electron microscope.<ref name="Ross and Pawlina, 2016" />

===Specialized techniques===

====Cryosectioning==== {{Main|Frozen section procedure}} Similar to the frozen section procedure employed in medicine, '''cryosectioning''' is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome.<ref name="Bancroft and Stevens, 1982" /> The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues. Unfixed frozen sections can be used for studies requiring enzyme localization in tissues and cells. Tissue fixation is required for certain procedures such as antibody-linked immunofluorescence staining. Frozen sections are often prepared during surgical removal of tumors to allow rapid identification of tumor margins, as in Mohs surgery, or determination of tumor malignancy, when a tumor is discovered incidentally during surgery.

====Ultramicrotomy==== [[Image:Chlamydomonas TEM 07.jpg|thumb|300px|right|Green algae under a Transmission electron microscope ]]

{{Main|Ultramicrotomy}}

Ultramicrotomy is a method of preparing extremely thin sections for transmission electron microscope (TEM) analysis. Tissues are commonly embedded in epoxy or other plastic resin.<ref name="Ross and Pawlina, 2016" /> Very thin sections (less than 0.1 micrometer in thickness) are cut using diamond or glass knives on an ultramicrotome.<ref name="Bancroft and Stevens, 1982" />

===Artifacts=== Artifacts are structures or features in tissue that interfere with normal histological examination. Artifacts interfere with histology by changing the tissues appearance and hiding structures. Tissue processing artifacts can include pigments formed by fixatives,<ref name="Bancroft and Stevens, 1982" /> shrinkage, washing out of cellular components, color changes in different tissues types and alterations of the structures in the tissue. An example is mercury pigment left behind after using Zenker's fixative to fix a section.<ref name="Bancroft and Stevens, 1982" /> Formalin fixation can also leave a brown to black pigment under acidic conditions.<ref name="Bancroft and Stevens, 1982" />

==History== [[File:Cajal-va.jpg|thumb|left|Santiago Ramón y Cajal in his laboratory]] In the 17th century the Italian Marcello Malpighi used microscopes to study tiny biological entities; some regard him as the founder of the fields of histology and microscopic pathology.<ref name="Bracegirdle, 1977" /><ref name="Motta 1998" /> Malpighi analyzed several parts of the organs of bats, frogs and other animals under the microscope. While studying the structure of the lung, Malpighi noticed its membranous alveoli and the hair-like connections between veins and arteries, which he named capillaries. His discovery established how the oxygen breathed in enters the blood stream and serves the body.<ref name="Adelmann and Malpighi, 1966" />

In the 19th century histology was an academic discipline in its own right. The French anatomist Xavier Bichat introduced the concept of tissue in anatomy in 1801,<ref name="Bichat, 1801" /> and the term "histology" ({{langx |de| Histologie}}), coined to denote the "study of tissues", first appeared in a book by Karl Mayer in 1819.<ref name="Mayer, 1891" /><ref name="bock" /><ref name="Bracegirdle, 1977" /> Bichat described twenty-one human tissues, which can be subsumed under the four categories currently accepted by histologists.<ref name="Rather, 1978" /> The usage of illustrations in histology, deemed as useless by Bichat, was promoted by Jean Cruveilhier.<ref> {{cite book | last = Meli | first = Domenico Bertoloni | name-list-style = vanc | year = 2017 | title = Visualizing disease: the art and history of pathological illustrations | location = Chicago | publisher = The University of Chicago Press }}{{page needed|date= August 2018}} </ref>{{when|date=June 2019}}

In the early 1830s Purkynĕ invented a microtome with high precision.<ref name=bock/>

During the 19th century many fixation techniques were developed by Adolph Hannover (solutions of chromates and chromic acid), Franz Schulze and Max Schultze (osmic acid), Alexander Butlerov (formaldehyde) and Benedikt Stilling (freezing).<ref name=bock/>

Mounting techniques were developed by Rudolf Heidenhain (1824–1898), who introduced gum Arabic; Salomon Stricker (1834–1898), who advocated a mixture of wax and oil; and Andrew Pritchard (1804–1884) who, in 1832, used a gum/isinglass mixture. In the same year, Canada balsam appeared on the scene, and in 1869 Edwin Klebs (1834–1913) reported that he had for some years embedded his specimens in paraffin.<ref>{{Cite journal|last=Bock|first=Ortwin|date=2015-01-05|title=A history of the development of histology up to the end of the nineteenth century|url=http://www.labome.org/research/A-history-of-the-development-of-histology-up-to-the-end-of-the-nineteenth-century.html|journal=Research|access-date=2018-05-03|archive-date=2021-04-13|archive-url=https://web.archive.org/web/20210413202849/http://www.labome.org/research/A-history-of-the-development-of-histology-up-to-the-end-of-the-nineteenth-century.html|url-status=dead}}</ref>

The 1906 Nobel Prize in Physiology or Medicine was awarded to histologists Camillo Golgi and Santiago Ramon y Cajal. They had conflicting interpretations of the neural structure of the brain based on differing interpretations of the same images. Ramón y Cajal won the prize for his correct theory, and Golgi for the silver-staining technique that he invented to make it possible.<ref name="NobelPrize1906" />

==Future directions== ===''In vivo'' histology=== There is interest in developing techniques for ''in vivo'' histology (predominantly using MRI), which would enable doctors to non-invasively gather information about healthy and diseased tissues in living patients, rather than from fixed tissue samples.<ref>{{Cite journal|last1=Dominietto|first1=Marco|last2=Rudin|first2=Markus|date=2014|title=Could magnetic resonance provide in vivo histology?|journal=Frontiers in Genetics|volume=4|page=298|doi=10.3389/fgene.2013.00298|issn=1664-8021|pmc=3888945|pmid=24454320|doi-access=free}}</ref><ref>{{Cite journal|last1=Delnoij|first1=Thijs|last2=van Suylen|first2=Robert Jan|last3=Cleutjens|first3=Jack P.M.|last4=Schalla|first4=Simon|last5=Bekkers|first5=Sebastiaan C.A.M.|date=October 2009|title=In vivo histology by cardiovascular magnetic resonance imaging|journal=European Heart Journal|language=en|volume=30|issue=20|pages=2492|doi=10.1093/eurheartj/ehp319|pmid=19696188|issn=1522-9645|doi-access=free}}</ref><ref>{{Cite journal|last1=Bridge|first1=Holly|last2=Clare|first2=Stuart|date=2006-01-29|title=High-resolution MRI: in vivo histology?|journal=Philosophical Transactions of the Royal Society B: Biological Sciences|language=en|volume=361|issue=1465|pages=137–146|doi=10.1098/rstb.2005.1777|issn=0962-8436|pmc=1626544|pmid=16553313}}</ref><ref>{{Cite journal|last1=Deistung|first1=Andreas|last2=Schäfer|first2=Andreas|last3=Schweser|first3=Ferdinand|last4=Biedermann|first4=Uta|last5=Turner|first5=Robert|last6=Reichenbach|first6=Jürgen R.|date=January 2013|title=Toward in vivo histology: A comparison of quantitative susceptibility mapping (QSM) with magnitude-, phase-, and R2⁎-imaging at ultra-high magnetic field strength|journal=NeuroImage|language=en|volume=65|pages=299–314|doi=10.1016/j.neuroimage.2012.09.055|pmid=23036448|s2cid=140122831}}</ref>

==See also== {{Portal|Medicine}} *National Society for Histotechnology *Slice preparation

==Notes== {{Reflist|group=help}}

== References == <references>

<ref name="Adelmann and Malpighi, 1966">{{cite book | last1 = Adelmann | first1 = Howard B | first2 = Marcello | last2 = Malpighi | name-list-style = vanc | date = 1966 | title = Marcello Malpighi and the Evolution of Embryology | volume = 5 | publisher = Cornell University Press | location = Ithaca, N.Y. | oclc = 306783 }}</ref>

<ref name="Bancroft and Stevens, 1982">{{cite book |editor1-last=Bancroft |editor1-first=John |editor2-last=Stevens |editor2-first=Alan |title=The Theory and Practice of Histological Techniques |date=1982 |publisher=Longman Group Limited |edition= 2nd }}</ref>

<ref name="Bennike et al., 2016">{{cite journal | vauthors = Bennike TB, Kastaniegaard K, Padurariu S, Gaihede M, Birkelund S, Andersen V, Stensballe A | title = Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples | journal = EuPA Open Proteomics | volume = 10 | pages = 9–18 | date = March 2016 | pmid = 29900094 | pmc = 5988570 | doi = 10.1016/j.euprot.2015.10.001 }}</ref>

<ref name=bock>{{cite journal |doi=10.13070/rs.en.2.1283 |last1=Bock |first1=Ortwin |name-list-style=vanc |year=2015 |title=A history of the development of histology up to the end of the nineteenth century |journal=Research |volume=2 |pages=1283 |url=http://www.labome.org/research/A-history-of-the-development-of-histology-up-to-the-end-of-the-nineteenth-century.html |doi-broken-date=12 July 2025 |access-date=3 May 2018 |archive-date=13 April 2021 |archive-url=https://web.archive.org/web/20210413202849/http://www.labome.org/research/A-history-of-the-development-of-histology-up-to-the-end-of-the-nineteenth-century.html |url-status=dead }}</ref>

<ref name="Bracegirdle, 1977">{{cite journal |doi=10.1177/007327537701500201 |title=The History of Histology: A Brief Survey of Sources |journal=History of Science |volume=15 |issue=2 |pages=77–101 |year=1977 |last1=Bracegirdle |first1=Brian | name-list-style = vanc |bibcode=1977HisSc..15...77B |s2cid=161338778 }}</ref>

<ref name="Canoville and Chinsamy, 2015">{{cite journal| author=Canoville A, Chinsamy A| title=Bone Microstructure of the Stereospondyl Lydekkerina Huxleyi Reveals Adaptive Strategies to the Harsh Post Permian-Extinction Environment. | journal=The Anatomical Record | year= 2015 | volume= 298 | issue= 7 | pages= 1237–54 | pmid=25857487 | doi=10.1002/ar.23160 | s2cid=43628074 | doi-access=free }}</ref>

<ref name="Dapson and Horobin, 2009">{{cite journal|vauthors=Dapson RW, Horobin RW| title=Dyes from a twenty-first century perspective. | journal=Biotech Histochem | year= 2009 | volume= 84 | issue= 4 | pages= 135–7 | pmid=19384743 | doi=10.1080/10520290902908802 | s2cid=28563610 }}</ref>

<ref name="Drury and Wallington, 1980">{{cite book |last1=Drury |first1=R. A. B. |last2=Wallington |first2=E. A. |title=Carleton's Histological Technique |date=1980 |publisher=Oxford University Press |isbn=0-19-261310-3 |pages=520 |edition=5th}}</ref>

<ref name="Leeson and Leeson, 1981">{{cite book |last1=Leeson |first1=Thomas S. |last2=Leeson |first2=C. Roland |title=Histology |date=1981 |publisher=W. B. Saunders Company |isbn=978-0721657042 |pages=600 |edition= Fourth}}</ref>

<ref name="Mayer, 1891">{{cite book | last = Mayer | first = August Franz Josef Karl | name-list-style = vanc | year = 1819 | title = Ueber Histologie und eine neue Eintheilung der Gewebe des menschlichen Körpers | location = Bonn | publisher = Adolph Marcus | url = https://archive.org/details/bub_gb_1B8_AAAAcAAJ | language = de }}</ref>

<ref name="Collins">{{cite web | title = Microanatomy definition and meaning | work = Collins English Dictionary | url = https://www.collinsdictionary.com/dictionary/english/microanatomy }}</ref>

<ref name="Bichat, 1801">{{cite book | vauthors = Bichat X | date = 1801 | chapter = Considérations générales | title = Anatomie générale appliquée à la physiologie et à la médecine | publisher = Chez Brosson, Gabon et Cie, Libraires, rue Pierre-Sarrazin, no. 7, et place de l'École de Médecine | location = Paris | chapter-url = https://archive.org/details/anatomiegnra001bich | pages = cvj–cxj | language = fr }}</ref>

<ref name="Weiss et al., 2011">{{cite journal | vauthors = Weiss AT, Delcour NM, Meyer A, Klopfleisch R | title = Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues | journal = Veterinary Pathology | volume = 48 | issue = 4 | pages = 834–8 | date = July 2011 | pmid = 20817894 | doi = 10.1177/0300985810380399 | s2cid = 34974790 | doi-access = free }}</ref>

<ref name="Maximow and Bloom, 1957">{{cite book |last1=Maximow |first1=Alexander A. |last2=Bloom |first2=William |title=A textbook of Histology |date=1957 |publisher=W. B. Saunders Company |location=Philadelphia |edition= Seventh}}</ref>

<ref name="Motta 1998">{{cite journal| author=Motta PM| title=Marcello Malpighi and the foundations of functional microanatomy. | journal=Anat Rec | year= 1998 | volume= 253 | issue= 1 | pages= 10–2 | pmid=9556019 | doi=10.1002/(SICI)1097-0185(199802)253:1<10::AID-AR7>3.0.CO;2-I | doi-access=free }}</ref>

<ref name="Padian and Lamm, 2013">{{cite book |editor1-last=Padian |editor1-first=Kevin |editor2-last=Lamm |editor2-first=Ellen-Thérèse |title=Bone histology of fossil tetrapods : Advancing methods, analysis, and interpretation |date=2013 |publisher=University of California Press |isbn=978-0-520-27352-8 |pages=298 |edition=1st}}</ref>

<ref name="Rather, 1978">{{cite book | vauthors = Rather LJ | year = 1978 | title = The Genesis of Cancer: A Study in the History of Ideas | location = Baltimore | publisher = Johns Hopkins University Press | quote =Most of Bichat's twenty-one tissues can be subsumed under the four categories generally accepted by contemporary histologists; epithelium, connective tissue, muscle, and nerve. Four of Bichat's tissues fall under the heading of epithelium (epidermoid, mucous, serous, and synovial); six under connective tissue (dermoid, fibrous, fibrocartilaginous, cartilaginous, osseous, and cellular); two under muscle; and two under nerve — the distinction between nervous governing "animal" life and nervous governing "organic" life corresponds with that between the voluntary and involuntary nervous systems. The arteries and the veins, long sources of contention, are classified today as compound tissues. The absorbents and the exhalants (which Bichat thought to be open-ended vessels) have dropped out or been replaced by the lymphatics. His medullary system has no counterpart among the present-day tissues. | url = https://archive.org/details/genesisofcancers0000rath | url-access = registration | isbn = 9780801821035 }}</ref>

<ref name="Rosai, 2007">{{cite journal| author=Rosai J| title=Why microscopy will remain a cornerstone of surgical pathology. | journal=Lab Invest | year= 2007 | volume= 87 | issue= 5 | pages= 403–8 | pmid=17401434 | doi=10.1038/labinvest.3700551 | s2cid=27399409 | doi-access=free }}</ref>

<ref name="Ross and Pawlina, 2016">{{cite book |last1=Ross |first1=Michael H. |last2=Pawlina |first2=Wojciech |title=Histology : a text and atlas : with correlated cell and molecular biology |date=2016 |publisher=Wolters Kluwer |isbn=978-1451187427 |pages=984p |edition=7th}}</ref>

<ref name="Stedman's medical dictionary">{{cite book |title=Stedman's medical dictionary. |publisher=Lippincott Williams & Wilkins |isbn=978-0683400076 |edition=27th |url=https://archive.org/details/stedmansmedicald00sted_3 |year=2006 }}</ref>

<ref name="Titford and Bowman, 2012">{{cite journal|last1=Titford|first1=Michael|last2=Bowman|first2=Blythe|title=What May the Future Hold for Histotechnologists?|journal=Laboratory Medicine|volume=43|issue=suppl 2|year=2012|pages=e5–e10|issn=0007-5027|doi=10.1309/LMXB668WDCBIAWJL|doi-access=free}}</ref>

<ref name="Defined terms histology">{{Cite news|url=https://definedterm.com/histology|title=DefinedTerm: Histology|work=Defined Term|access-date=2018-10-29|language=en-US|archive-date=2018-10-29|archive-url=https://web.archive.org/web/20181029191818/https://definedterm.com/histology|url-status=dead}}</ref>

<ref name="Britannica histology">{{Cite encyclopedia|url=https://www.britannica.com/science/histology|title=Histology {{!}} physiology|encyclopedia=Encyclopedia Britannica|access-date=2018-10-29|language=en}}</ref>

<ref name="Wick, 2019">{{cite journal|last1=Wick|first1=Mark R.|title=The hematoxylin and eosin stain in anatomic pathology—An often-neglected focus of quality assurance in the laboratory|journal=Seminars in Diagnostic Pathology|volume=36|issue=5|pages=303–311|year=2019|issn=0740-2570|doi=10.1053/j.semdp.2019.06.003|pmid=31230963|s2cid=195326749}}</ref>

<ref name="NobelPrize1906">{{cite web | url = https://www.nobelprize.org/prizes/medicine/1906/summary/ | title = The Nobel Prize in Physiology or Medicine 1906 | website = NobelPrize.org}}</ref> </references>

==External links== *{{Commons category-inline|Histology}}

{{Epithelium and epithelial tissue}} {{Branches of biology}} {{Biology_nav}} {{Connective tissue}} {{Muscle tissue}} {{Nervous tissue}} {{Authority control}}

Category:Histology Category:Histotechnology Category:Staining Category:Histochemistry Category:Anatomy Category:Laboratory health care occupations