'''Fluorogenic''' describes a property of chemical compounds which are initially not fluorescent, but become fluorescent through a chemical reaction, typically through an intermolecular covalent reaction which binds the now fluorescent compound to a target molecule.<ref name="McChesney-Harris Kakodakar Bernstein Stobaugh 2012 pp. 117–132">{{cite journal | last1=McChesney-Harris | first1=L. L. | last2=Kakodakar | first2=S. V. | last3=Bernstein | first3=S. C. | last4=Stobaugh | first4=J. F. | title=Mechanism Based Design of Amine Fluorogenic Derivatization Reagents: Proof of Concept, Physical–Chemical Characterization and Initial Analytical Derivatization Protocols | journal=Chromatographia | publisher=Springer Science and Business Media LLC | volume=76 | issue=3–4 | date=2012-12-22 | issn=0009-5893 | doi=10.1007/s10337-012-2371-9 | pages=117–132| s2cid=95016112 }}</ref> IUPAC uses a broader definition of fluorogenic, wherein an enhancement of fluorescence via a chemical reaction is not required, however in contrast to the IUPAC definition common use of fluorogenic does not refer to non-reaction effects like the enhancement of fluorescence from a fluorophore being in different solvents.<ref>{{cite journal |title=Fluorogenic |url=https://goldbook.iupac.org/terms/view/FT07379 |website=IUPAC GoldBook |year=2014 |publisher=IUPAC |doi=10.1351/goldbook.FT07379 |access-date=10 March 2023|doi-access=free |url-access=subscription }}</ref> Fluorogenic labeling reagents are often used in analytical chemistry procedures, particularly in HPLC or CE to derivative target compounds (e.g. labeling the primary amines of polypeptides), thereby allowing enhanced sensitivity through fluorescence based detection.<ref name="Fanali Haddad Poole Riekkola 2017 p. ">{{cite book | last1=Fanali | first1=Salvatore | last2=Haddad | first2=Paul R. | last3=Poole | first3=Colin | last4=Riekkola | first4=Marja-Liisa | title=Liquid chromatography. Volume 2, Applications | publication-place=Amsterdam, Netherlands | date=2017 | isbn=978-0-12-809344-3 | oclc=992119225 | language=en | page=}}</ref>
==Examples== * OPA * Fluorescamine * FQ * NBD-F * 6-AQC * Epicocconone * CBQCA
==See also== * Colorogenic
==References== {{reflist}}
Category:Fluorescence Category:Analytical chemistry
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