{{Infobox protein family | Symbol = Ydc2-catalyst | Name = Ydc2 protein domain | image = PDB 1kcf EBI.jpg | width = | caption = Crystal structure of the yeast mitochondrial Holliday junction resolvase, YDC2 | Pfam = PF09159 | Pfam_clan = CL0219 | InterPro = IPR015242 | SMART = | PROSITE = | MEROPS = | SCOP = 1kcf | TCDB = | OPM family = | OPM protein = | CAZy = | CDD = cd00529 }} In molecular biology, the protein domain, '''Ydc2''' (also known as SpCce1), is a Holliday junction resolvase from the fission yeast ''Schizosaccharomyces pombe'' that is involved in the maintenance of mitochondrial DNA.
==Function== The Ydc2 domains are enzymes (or "biological catalysts") that resolve Holliday junctions into separate DNA duplexes by cleaving DNA after 5'-CT-3, and 5'-TT-3, sequences.
==Properties== The junction resolving enzymes are very diverse, but have the following properties in common: *high structure specificity for binding *metal dependent, sequence specific cleavage activity<ref name="pmid9837990">{{cite journal|vauthors=White MF, Lilley DM | title=Interaction of the resolving enzyme YDC2 with the four-way DNA junction. | journal=Nucleic Acids Res | year= 1998 | volume= 26 | issue= 24 | pages= 5609–16 | pmid=9837990 | doi= 10.1093/nar/26.24.5609| pmc=148026 }}</ref> Essentially, they are highly specific.
==Limiting factors== Furthermore, the cleavage efficiency is affected by: *strand type (continuous or exchange) *nucleotide sequence at cleavage site<ref name="pmid9837990"/>
==Structure== This protein domain forms a ribonuclease H fold consisting of two beta sheets and one alpha helix, arranged as a beta-alpha-beta motif. Each beta sheet has five strands, arranged in a 32145 order, with the second strand being antiparallel to the rest.<ref name="pmid11726496">{{cite journal |vauthors=Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE | title = Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2 | journal = EMBO J. | volume = 20 | issue = 23 | pages = 6601–11 |date=December 2001 | pmid = 11726496 | pmc = 125760 | doi = 10.1093/emboj/20.23.6601 }}</ref>
==References== {{reflist}} {{InterPro content|IPR015242}}
Category:Protein domains