YEPD — Mediated Wiki

{{short description|Medium for yeast growth}} thumb|right|200px|YPD medium bottle and YPD agar plate '''YEPD''' or '''yeast extract peptone dextrose''', also often abbreviated as '''YPD''', is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose).<ref>{{Cite journal|date=2010-09-01|title=YPD media|url=http://cshprotocols.cshlp.org/content/2010/9/pdb.rec12315|journal=Cold Spring Harbor Protocols|language=en|volume=2010|issue=9|article-number=pdb.rec12315|doi=10.1101/pdb.rec12315|issn=1940-3402|url-access=subscription}}</ref> It can be used as solid medium by including agar. The yeast extract will typically contain all the amino acids necessary for growth. By being a ''complete'' medium, YEPD cannot be used as a selection medium to test for auxotrophs. Instead, YEPD is used as a growth medium to grow yeast cultures.

YEPD typically contains 1% yeast extract, 2% peptone, and 2% glucose in distilled water. It may be made as a broth, or made into an agar gel by adding 1.5 to 2% agar.<ref>{{Cite web|url=http://www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/242820.pdf|title=Yeast Extract-Peptone-Dextrose (YPD) Agar|access-date=2017-12-29|archive-date=2017-12-29|archive-url=https://web.archive.org/web/20171229233058/http://www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/242820.pdf|url-status=live}}</ref><ref>{{Cite web|url=http://fg.cns.utexas.edu/fg/protocol:_YPD_plates.html|title=protocol: YPD plates|website=fg.cns.utexas.edu|language=en|access-date=2017-12-29|archive-date=2010-03-03|archive-url=https://web.archive.org/web/20100303015834/http://fg.cns.utexas.edu/fg/protocol:_YPD_plates.html}}</ref>

==References== {{reflist}}

Category:Microbiological media