Vesicle-associated membrane protein 8

{{Short description|Protein-coding gene in the species Homo sapiens}} {{cs1 config|name-list-style=vanc}} {{Infobox_gene}} '''Vesicle-associated membrane protein 8''' is a protein that in humans is encoded by the ''VAMP8'' gene.<ref name="pmid9878266">{{cite journal |vauthors=Bui TD, Wong SH, Lu L, Hong W | title = Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse | journal = Genomics | volume = 54 | issue = 3 | pages = 579–80 |date=February 1999 | pmid = 9878266 | doi = 10.1006/geno.1998.5596 }}</ref><ref name="pmid9614193">{{cite journal |vauthors=Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W | title = Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome | journal = Mol Biol Cell | volume = 9 | issue = 6 | pages = 1549–63 |date=July 1998 | pmid = 9614193 | pmc = 25382 | doi = 10.1091/mbc.9.6.1549}}</ref><ref name="entrez">{{cite web | title = Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)| url = https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=8673}}</ref>

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex.<ref name="entrez" /> Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion.<ref name="pmid27402227">{{cite journal |vauthors=Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T | title = Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion | journal = EMBO Journal | volume = 35 | issue = 16 | pages = 1721–1843 |date=July 2016 | pmid = 27402227 | pmc = 5010044 | doi = 10.15252/embj.201694071 }}</ref>

In pancreatic β-cells, VAMP8 has been shown to be part of the endosomal system. It is particularly localized to Rab11 recycling endosomes, where it plays an important role in GLP1R and GLUT2 recycling. Overexpression of VAMP8 in β-cells results in decreased insulin secretion.<ref>{{Cite journal |last=Liu |first=Liangwen |last2=Marshall |first2=Misty |last3=Chadeuf |first3=Emmanuel |last4=Saras |first4=Jan |last5=Barg |first5=Sebastian |date=2025-09-01 |title=VAMP8 Is an Endosomal v-SNARE That Supports GLP-1 Receptor Recycling in Pancreatic β-Cells |url=https://diabetesjournals.org/diabetes/article/74/9/1577/162865/VAMP8-Is-an-Endosomal-v-SNARE-That-Supports-GLP-1 |journal=Diabetes |language=en |volume=74 |issue=9 |pages=1577–1588 |doi=10.2337/db24-0921 |issn=0012-1797 |pmc=12365419 |pmid=40608311}}</ref>

==Interactions== Vesicle-associated membrane protein 8 has been shown to interact with STX4,<ref name=pmid10820264>{{cite journal |last=Paumet |first=F |author2=Le Mao J |author3=Martin S |author4=Galli T |author5=David B |author6=Blank U |author7=Roa M |date=June 2000 |title=Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment |journal=J. Immunol. |volume=164 |issue=11 |pages=5850–7 | issn = 0022-1767| pmid = 10820264 | doi=10.4049/jimmunol.164.11.5850|doi-access=free }}</ref><ref name=pmid12130530>{{cite journal |last=Polgár |first=János |author2=Chung Sul-Hee |author3=Reed Guy L |date=August 2002 |title=Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion |journal=Blood |volume=100 |issue=3 |pages=1081–3 | issn = 0006-4971| pmid = 12130530 |doi=10.1182/blood.V100.3.1081 |s2cid=36597939 |doi-access= }}</ref> SNAP23,<ref name=pmid10820264/><ref name=pmid12828989>{{cite journal |last=Imai |first=Akane |author2=Nashida Tomoko |author3=Yoshie Sumio |author4=Shimomura Hiromi |date=August 2003 |title=Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane |journal=Arch. Oral Biol. |volume=48 |issue=8 |pages=597–604 | issn = 0003-9969| pmid = 12828989 |doi=10.1016/S0003-9969(03)00116-X }}</ref> STX1A,<ref name=pmid11112705>{{cite journal |last=Nagamatsu |first=S |author2=Nakamichi Y |author3=Watanabe T |author4=Matsushima S |author5=Yamaguchi S |author6=Ni J |author7=Itagaki E |author8=Ishida H |date=January 2001 |title=Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules |journal=J. Cell Sci. |volume=114 |issue=Pt 1 |pages=219–227 |doi=10.1242/jcs.114.1.219 | issn = 0021-9533| pmid = 11112705 }}</ref> STX8<ref name=pmid11101518>{{cite journal |last=Antonin |first=W |author2=Holroyd C |author3=Fasshauer D |author4=Pabst S |author5=Von Mollard G F |author6=Jahn R |date=Dec 2000 |title=A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function |journal=EMBO J. |volume=19 |issue=23 |pages=6453–64 | issn = 0261-4189| pmid = 11101518 |doi = 10.1093/emboj/19.23.6453 |pmc=305878 }}</ref> and STX7.<ref name=pmid11101518/><ref name=pmid11278762>{{cite journal |last=Wade |first=N |author2=Bryant N J |author3=Connolly L M |author4=Simpson R J |author5=Luzio J P |author6=Piper R C |author7=James D E |date=June 2001 |title=Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells |journal=J. Biol. Chem. |volume=276 |issue=23 |pages=19820–7 | issn = 0021-9258| pmid = 11278762 |doi = 10.1074/jbc.M010838200 |doi-access=free }}</ref>

==References== {{Reflist}}

==Further reading== {{Refbegin | 2}} *{{cite journal |vauthors=Bonaldo MF, Lennon G, Soares MB |title=Normalization and subtraction: two approaches to facilitate gene discovery |journal=Genome Res. |volume=6 |issue= 9 |pages= 791–806 |year= 1997 |pmid= 8889548 |doi=10.1101/gr.6.9.791 |doi-access=free }} *{{cite journal | author=Fasshauer D |title=Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties |journal=J. Biol. Chem. |volume=274 |issue= 22 |pages= 15440–6 |year= 1999 |pmid= 10336434 |doi=10.1074/jbc.274.22.15440 | author2=Antonin W | author3=Margittai M | display-authors=3 | last4=Pabst | first4=S | last5=Jahn | first5=R |doi-access=free}} *{{cite journal | author=Kim PK |title=Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins |journal=J. Biol. Chem. |volume=274 |issue= 52 |pages= 36876–82 |year= 2000 |pmid= 10601239 |doi=10.1074/jbc.274.52.36876 | author2=Hollerbach C | author3=Trimble WS | display-authors=3 | last4=Leber | first4=B | last5=Andrews | first5=DW |doi-access=free}} *{{cite journal | author=Paumet F |title=Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment |journal=J. Immunol. |volume=164 |issue= 11 |pages= 5850–7 |year= 2000 |pmid= 10820264 |doi= 10.4049/jimmunol.164.11.5850| author2=Le Mao J | author3=Martin S | display-authors=3 | last4=Galli | first4=T | last5=David | first5=B | last6=Blank | first6=U | last7=Roa | first7=M | doi-access=free }} *{{cite journal | author=Antonin W |title=The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes |journal=Mol. Biol. Cell |volume=11 |issue= 10 |pages= 3289–98 |year= 2000 |pmid= 11029036 |doi= 10.1091/mbc.11.10.3289| pmc=14992 | author2=Holroyd C | author3=Tikkanen R | display-authors=3 | last4=Höning | first4=S | last5=Jahn | first5=R }} *{{cite journal | author=Antonin W |title=A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function |journal=EMBO J. |volume=19 |issue= 23 |pages= 6453–64 |year= 2001 |pmid= 11101518 |doi= 10.1093/emboj/19.23.6453 | pmc=305878 | author2=Holroyd C | author3=Fasshauer D | display-authors=3 | last4=Pabst | first4=S | last5=Von Mollard | first5=GF | last6=Jahn | first6=R }} *{{cite journal | author=Nagamatsu S |title=Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules |journal=J. Cell Sci. |volume=114 |issue= Pt 1 |pages= 219–227 |year= 2001 |pmid= 11112705 | author2=Nakamichi Y | author3=Watanabe T | display-authors=3 | last4=Matsushima | first4=S | last5=Yamaguchi | first5=S | last6=Ni | first6=J | last7=Itagaki | first7=E | last8=Ishida | first8=H |doi=10.1242/jcs.114.1.219 }} *{{cite journal | author=Wade N |title=Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells |journal=J. Biol. Chem. |volume=276 |issue= 23 |pages= 19820–7 |year= 2001 |pmid= 11278762 |doi= 10.1074/jbc.M010838200 | author2=Bryant NJ | author3=Connolly LM | display-authors=3 | last4=Simpson | first4=RJ | last5=Luzio | first5=JP | last6=Piper | first6=RC | last7=James | first7=DE | doi-access=free }} *{{cite journal | author=Antonin W |title=Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs |journal=Nat. Struct. Biol. |volume=9 |issue= 2 |pages= 107–11 |year= 2002 |pmid= 11786915 |doi= 10.1038/nsb746 | author2=Fasshauer D | author3=Becker S | display-authors=3 | last4=Jahn | first4=Reinhard | last5=Schneider | first5=Thomas R. |hdl=11858/00-001M-0000-0012-F459-F |s2cid=17724790 |hdl-access=free }} *{{cite journal |vauthors=Polgár J, Chung SH, Reed GL |title=Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion |journal=Blood |volume=100 |issue= 3 |pages= 1081–3 |year= 2002 |pmid= 12130530 |doi=10.1182/blood.V100.3.1081 |s2cid=36597939 |doi-access= }} *{{cite journal | author=Strausberg RL |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 | pmc=139241 | author2=Feingold EA | author3=Grouse LH | display-authors=3 | last4=Derge | first4=JG | last5=Klausner | first5=RD | last6=Collins | first6=FS | last7=Wagner | first7=L | last8=Shenmen | first8=CM | last9=Schuler | first9=GD |bibcode=2002PNAS...9916899M|doi-access=free }} *{{cite journal |vauthors=Imai A, Nashida T, Yoshie S, Shimomura H |title=Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane |journal=Arch. Oral Biol. |volume=48 |issue= 8 |pages= 597–604 |year= 2003 |pmid= 12828989 |doi=10.1016/S0003-9969(03)00116-X }} *{{cite journal | author=Gerhard DS |title=The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121–7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 | pmc=528928 | author2=Wagner L | author3=Feingold EA | display-authors=3 | last4=Shenmen | first4=CM | last5=Grouse | first5=LH | last6=Schuler | first6=G | last7=Klein | first7=SL | last8=Old | first8=S | last9=Rasooly | first9=R }} *{{cite journal | author=Hillier LW |title=Generation and annotation of the DNA sequences of human chromosomes 2 and 4 |journal=Nature |volume=434 |issue= 7034 |pages= 724–31 |year= 2005 |pmid= 15815621 |doi= 10.1038/nature03466 | author2=Graves TA | author3=Fulton RS | display-authors=3 | last4=Fulton | first4=Lucinda A. | last5=Pepin | first5=Kymberlie H. | last6=Minx | first6=Patrick | last7=Wagner-Mcpherson | first7=Caryn | last8=Layman | first8=Dan | last9=Wylie | first9=Kristine |bibcode=2005Natur.434..724H| doi-access=free }} *{{cite journal | author=Brinkman JF |title=VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma |journal=Mol. Vis. |volume=11 |pages= 582–6 |year= 2006 |pmid= 16110299 | author2=Ottenheim CP | author3=de Jong LA | display-authors=3 | last4=Zegers | first4=RH | last5=De Smet | first5=MD | last6=De Jong | first6=PT | last7=Bergen | first7=AA }} *{{cite journal | author=Oishi Y |title=Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells |journal=Histochem. Cell Biol. |volume=125 |issue= 3 |pages= 273–81 |year= 2007 |pmid= 16195891 |doi= 10.1007/s00418-005-0068-y | author2=Arakawa T | author3=Tanimura A | display-authors=3 | last4=Itakura | first4=Makoto | last5=Takahashi | first5=Masami | last6=Tajima | first6=Yoshifumi | last7=Mizoguchi | first7=Itaru | last8=Takuma | first8=Taishin |s2cid=10541252 }} *{{cite journal | author=Shiffman D |title=Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction |journal=Arterioscler. Thromb. Vasc. Biol. |volume=26 |issue= 7 |pages= 1613–8 |year= 2006 |pmid= 16690874 |doi= 10.1161/01.ATV.0000226543.77214.e4 | author2=Rowland CM | author3=Louie JZ | display-authors=3 | last4=Luke | first4=MM | last5=Bare | first5=LA | last6=Bolonick | first6=JI | last7=Young | first7=BA | last8=Catanese | first8=JJ | last9=Stiggins | first9=CF | doi-access=free }} {{Refend}} {{PDB Gallery|geneid=8673}}