Selector technique

{{Single source|date=December 2025}} The '''selector technique''' is a method to amplify and multiplex genomic DNA.<ref name="Isaksson">{{Cite journal|url=https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-121429|title=Extracting Genomic Variations using Selector Technology|first=Magnus|last=Isaksson|date=December 21, 2010|via=www.diva-portal.org|journal=Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine}}</ref>

== Process == Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA.

==Selector construct==

A selector consists of two oligonucleotides, one '''Vector''' oligonucleotide and one '''Selector probe'''.<ref name="Isaksson" /> Together they form one '''Selector''' with target specific ends on each side of a general primer motif.

==Selection mechanisms==

# A selector probe hybridizes with both ends of the selected target. # A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using Taq polymerase.

==References== {{reflist}}

==Publications== *[https://web.archive.org/web/20051127101236/http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method] *[https://archive.today/20130415163620/http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments]

{{DEFAULTSORT:Selector-Technique}} Category:DNA