{{cs1 config |name-list-style=vanc}} {{advertisement|date=February 2026}} {{expert|Chemicals|date=February 2026}} '''SYTOX''' (also known as '''SYTOX Green''') is a high-affinity nucleic acid stain developed by Molecular Probes. Because the stain only penetrates cells with compromised plasma membranes, it can be used to investigate antibacterial mechanism of action<ref>{{Cite journal |vauthors=Lee H, Woo ER, Lee DG |date=July 2015 |title=Glochidioboside Kills Pathogenic Bacteria by Membrane Perturbation |journal=Current Microbiology |volume=71 |issue=1 |pages=1–7 |doi=10.1007/s00284-015-0807-9 |pmid=25820208 |s2cid=14011127}}</ref> and confirm loss of bacterial viability.<ref name="GaforioSerrano2002">{{Cite journal |vauthors=Gaforio JJ, Serrano MJ, Ortega E, Algarra I, Alvarez de Cienfuegos G |date=June 2002 |title=Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis |journal=Cytometry |volume=48 |issue=2 |pages=93–96 |doi=10.1002/cyto.10107 |pmid=12116370}}</ref><ref>{{Cite journal |vauthors=Lebaron P, Catala P, Parthuisot N |date=July 1998 |title=Effectiveness of SYTOX Green stain for bacterial viability assessment |journal=Applied and Environmental Microbiology |volume=64 |issue=7 |pages=2697–2700 |bibcode=1998ApEnM..64.2697L |doi=10.1128/AEM.64.7.2697-2700.1998 |pmc=106447 |pmid=9647851}}</ref> It can be used for live cell imaging of bacteria.<ref>{{Cite journal |vauthors=Bakshi S, Choi H, Rangarajan N, Barns KJ, Bratton BP, Weisshaar JC |date=August 2014 |title=Nonperturbative imaging of nucleoid morphology in live bacterial cells during an antimicrobial peptide attack |journal=Applied and Environmental Microbiology |volume=80 |issue=16 |pages=4977–4986 |bibcode=2014ApEnM..80.4977B |doi=10.1128/AEM.00989-14 |pmc=4135745 |pmid=24907320 |veditors=Parales RE}}</ref>
SYBR Safe, SYBR Green, PicoGreen, SYTO-16, SYTO-9 and TOPhBu all can be used to detect DNA at low concentrations was evaluated using two new metrics, absolute fluorescence enhancement (AFE) and relativefluorescence enhancement (RFE).<ref name=" Comprehensive Study of Thiazole-Orange-Based DNA Dyes ">{{Cite journal |last1=Domahidy |first1=Farkas |last2=Kovács |first2=Beatrix |last3=Cseri |first3=Levente |last4=Katona |first4=Gergely |last5=Rózsa |first5=Balázs |last6=Mucsi |first6=Zoltán |last7=Kovács |first7=Ervin |last8=Kovacs |first8=Ervin |date=2024-06-19 |title=Comprehensive Study of Thiazole-Orange-Based DNA Dyes |journal=ChemPhotoChem |language=en |article-number=e202400080 |doi=10.1002/cptc.202400080 |issn=2367-0932 |doi-access=free}}</ref>
== References == {{reflist}}
Category:Staining dyes Category:Cyanine dyes