S-phase kinase-associated protein 1

{{Short description|Protein-coding gene in the species Homo sapiens}} {{cs1 config|name-list-style=vanc}} {{Infobox_gene}} '''S-phase kinase-associated protein 1 ''' is an enzyme that in humans is encoded by the ''SKP1'' gene.<ref name="pmid7553852">{{cite journal | vauthors = Zhang H, Kobayashi R, Galaktionov K, Beach D | title = p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase | journal = Cell | volume = 82 | issue = 6 | pages = 915–25 | date = October 1995 | pmid = 7553852 | doi = 10.1016/0092-8674(95)90271-6 | s2cid = 16325791 | doi-access = free }}</ref><ref name="pmid8646875">{{cite journal | vauthors = Demetrick DJ, Zhang H, Beach DH | title = Chromosomal mapping of the genes for the human CDK2/cyclin A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2) | journal = Cytogenet Cell Genet | volume = 73 | issue = 1–2 | pages = 104–7 | date = July 1996 | pmid = 8646875 | doi = 10.1159/000134318 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: SKP1A S-phase kinase-associated protein 1A (p19A)| url = https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=6500}}</ref>

This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins containing an F-box motif), such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis.<ref name="Bai">{{cite journal | vauthors = Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ | title = SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. | journal = Cell | volume = 86 | issue = 2 | pages = 263–74 | year = 1996 | pmid = 8706131 | doi = 10.1016/S0092-8674(00)80098-7 | s2cid = 18387009 | doi-access = free }}</ref> The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

== Interactions ==

SKP1A has been shown to interact with: {{div col|colwidth=20em}} * BTRC,<ref name = pmid10531035/><ref name = pmid12609982/><ref name = pmid10918611/><ref name = pmid10644755>{{cite journal | vauthors = Suzuki H, Chiba T, Suzuki T, Fujita T, Ikenoue T, Omata M, Furuichi K, Shikama H, Tanaka K | title = Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination | journal = J. Biol. Chem. | volume = 275 | issue = 4 | pages = 2877–84 | date = January 2000 | pmid = 10644755 | doi = 10.1074/jbc.275.4.2877| doi-access = free }}</ref><ref name = pmid9660940>{{cite journal | vauthors = Margottin F, Bour SP, Durand H, Selig L, Benichou S, Richard V, Thomas D, Strebel K, Benarous R | title = A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif | journal = Mol. Cell | volume = 1 | issue = 4 | pages = 565–74 | date = March 1998 | pmid = 9660940 | doi = 10.1016/s1097-2765(00)80056-8| doi-access = free }}</ref> * CACYBP,<ref name = pmid11389839>{{cite journal | vauthors = Matsuzawa SI, Reed JC | title = Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses | journal = Mol. Cell | volume = 7 | issue = 5 | pages = 915–26 | date = May 2001 | pmid = 11389839 | doi = 10.1016/s1097-2765(01)00242-8| doi-access = free }}</ref> * CDCA3,<ref name = pmid12679038>{{cite journal | vauthors = Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW | title = Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC | journal = Cell | volume = 113 | issue = 1 | pages = 101–13 | date = April 2003 | pmid = 12679038 | doi = 10.1016/s0092-8674(03)00232-0| s2cid = 14962502 | doi-access = free | citeseerx = 10.1.1.522.1877 }}</ref> * CDK9,<ref name = pmid11689688>{{cite journal | vauthors = Kiernan RE, Emiliani S, Nakayama K, Castro A, Labbé JC, Lorca T, Nakayama Ki K, Benkirane M | title = Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome | journal = Mol. Cell. Biol. | volume = 21 | issue = 23 | pages = 7956–70 | date = Dec 2001 | pmid = 11689688 | pmc = 99964 | doi = 10.1128/MCB.21.23.7956-7970.2001 }}</ref> * CUL1,<ref name = pmid12609982/><ref name = pmid12628165>{{cite journal | vauthors = Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A | title = Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity | journal = Neuron | volume = 37 | issue = 5 | pages = 735–49 | date = March 2003 | pmid = 12628165 | doi = 10.1016/s0896-6273(03)00084-9| s2cid = 17024826 | doi-access = free }}</ref><ref name = pmid9430629>{{cite journal | vauthors = Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W | title = Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway | journal = EMBO J. | volume = 17 | issue = 2 | pages = 368–83 | date = January 1998 | pmid = 9430629 | pmc = 1170388 | doi = 10.1093/emboj/17.2.368 }}</ref><ref name = pmid11961546>{{cite journal | vauthors = Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P, Chu C, Koepp DM, Elledge SJ, Pagano M, Conaway RC, Conaway JW, Harper JW, Pavletich NP | title = Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex | journal = Nature | volume = 416 | issue = 6882 | pages = 703–9 | date = April 2002 | pmid = 11961546 | doi = 10.1038/416703a | bibcode = 2002Natur.416..703Z | s2cid = 4423882 }}</ref><ref name = pmid10230407>{{cite journal | vauthors = Ohta T, Michel JJ, Schottelius AJ, Xiong Y | title = ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity | journal = Mol. Cell | volume = 3 | issue = 4 | pages = 535–41 | date = April 1999 | pmid = 10230407 | doi = 10.1016/s1097-2765(00)80482-7| s2cid = 19371828 | doi-access = free }}</ref> * FBXL3,<ref name = pmid10531035>{{cite journal | vauthors = Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M | title = Identification of a family of human F-box proteins | journal = Curr. Biol. | volume = 9 | issue = 20 | pages = 1177–9 | date = October 1999 | pmid = 10531035 | doi = 10.1016/S0960-9822(00)80020-2 | s2cid = 7467493 | doi-access = free | bibcode = 1999CBio....9.1177C }}</ref> * FBXO4,<ref name = pmid10531035/><ref name = pmid17353931>{{cite journal | vauthors = Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D | title = Large-scale mapping of human protein-protein interactions by mass spectrometry | journal = Mol. Syst. Biol. | volume = 3 | pages = 89 | pmid = 17353931 | pmc = 1847948 | doi = 10.1038/msb4100134 | year=2007}}</ref> * FBXO5,<ref name = pmid10531035/> * FBXO7,<ref name = pmid10531035/> * FBXW2,<ref name = pmid10531035/> * FBXW7,<ref name = pmid12628165/><ref name = pmid11585921>{{cite journal | vauthors = Wu G, Lyapina S, Das I, Li J, Gurney M, Pauley A, Chui I, Deshaies RJ, Kitajewski J | title = SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation | journal = Mol. Cell. Biol. | volume = 21 | issue = 21 | pages = 7403–15 | date = November 2001 | pmid = 11585921 | pmc = 99913 | doi = 10.1128/MCB.21.21.7403-7415.2001 }}</ref> and * SKP2.<ref name = pmid10531035/><ref name = pmid12609982>{{cite journal | vauthors = Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB | title = TIP120A associates with cullins and modulates ubiquitin ligase activity | journal = J. Biol. Chem. | volume = 278 | issue = 18 | pages = 15905–10 | date = May 2003 | pmid = 12609982 | doi = 10.1074/jbc.M213070200 | doi-access = free }}</ref><ref name = pmid10918611>{{cite journal | vauthors = Strack P, Caligiuri M, Pelletier M, Boisclair M, Theodoras A, Beer-Romero P, Glass S, Parsons T, Copeland RA, Auger KR, Benfield P, Brizuela L, Rolfe M | title = SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4 | journal = Oncogene | volume = 19 | issue = 31 | pages = 3529–36 | date = July 2000 | pmid = 10918611 | doi = 10.1038/sj.onc.1203647 | doi-access = | s2cid = 24267499 }}</ref><ref name = pmid9827542>{{cite journal | vauthors = Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY | title = Characterization of the cullin and F-box protein partner Skp1 | journal = FEBS Lett. | volume = 438 | issue = 3 | pages = 183–9 | date = November 1998 | pmid = 9827542 | doi = 10.1016/s0014-5793(98)01299-x| s2cid = 40950881 | doi-access = free }}</ref><ref name = pmid11099048>{{cite journal | vauthors = Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS, Elledge SJ, Harper JW, Pagano M, Pavletich NP | title = Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex | journal = Nature | volume = 408 | issue = 6810 | pages = 381–6 | date = November 2000 | pmid = 11099048 | doi = 10.1038/35042620 | bibcode = 2000Natur.408..381S | s2cid = 4300503 }}</ref><ref name = pmid10559858>{{cite journal | vauthors = Marti A, Wirbelauer C, Scheffner M, Krek W | title = Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation | journal = Nat. Cell Biol. | volume = 1 | issue = 1 | pages = 14–9 | date = May 1999 | pmid = 10559858 | doi = 10.1038/8984 | s2cid = 8884226 }}</ref> {{Div col end}}

== References == {{Reflist|2}}

== Further reading == {{Refbegin | 2}} * {{cite journal | vauthors = Chen H, Thalmann I, Adams JC, Avraham KB, Copeland NG, Jenkins NA, Beier DR, Corey DP, Thalmann R, Duyk GM | title = cDNA cloning, tissue distribution, and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. | journal = Genomics | volume = 27 | issue = 3 | pages = 389–98 | year = 1995 | pmid = 7558018 | doi = 10.1006/geno.1995.1068 }} * {{cite journal | vauthors = Sowden J, Morrison K, Schofield J, Putt W, Edwards Y | title = A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l). | journal = Genomics | volume = 29 | issue = 1 | pages = 145–51 | year = 1996 | pmid = 8530064 | doi = 10.1006/geno.1995.1225 }} * {{cite journal | vauthors = Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ | title = SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. | journal = Cell | volume = 86 | issue = 2 | pages = 263–74 | year = 1996 | pmid = 8706131 | doi = 10.1016/S0092-8674(00)80098-7 | s2cid = 18387009 | doi-access = free }} * {{cite journal | vauthors = Brown KA, Leek JP, Lench NJ, Moynihan LM, Markham AF, Mueller RF | title = Human sequences homologous to the gene for the cochlear protein Ocp-II do not map to currently known non-syndromic hearing loss loci. | journal = Ann. Hum. Genet. | volume = 60 | issue = Pt 5 | pages = 385–9 | year = 1996 | pmid = 8912791 | doi = 10.1111/j.1469-1809.1996.tb00436.x | s2cid = 7687393 }} * {{cite journal | vauthors = Liang Y, Chen H, Asher JH, Chang CC, Friedman TB | title = Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter. | journal = Gene | volume = 184 | issue = 2 | pages = 163–7 | year = 1997 | pmid = 9031623 | doi = 10.1016/S0378-1119(96)00590-2 }} * {{cite journal | vauthors = Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W | title = Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway. | journal = EMBO J. | volume = 17 | issue = 2 | pages = 368–83 | year = 1998 | pmid = 9430629 | pmc = 1170388 | doi = 10.1093/emboj/17.2.368 }} * {{cite journal | vauthors = Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY | title = Characterization of the cullin and F-box protein partner Skp1. | journal = FEBS Lett. | volume = 438 | issue = 3 | pages = 183–9 | year = 1998 | pmid = 9827542 | doi = 10.1016/S0014-5793(98)01299-X | s2cid = 40950881 | doi-access = free }} * {{cite journal | vauthors = Yam CH, Ng RW, Siu WY, Lau AW, Poon RY | title = Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein Skp2. | journal = Mol. Cell. Biol. | volume = 19 | issue = 1 | pages = 635–45 | year = 1999 | pmid = 9858587 | pmc = 83921 | doi = 10.1128/mcb.19.1.635}} * {{cite journal | vauthors = Henzl MT, Thalmann I, Thalmann R | title = OCP2 exists as a dimer in the organ of Corti. | journal = Hear. Res. | volume = 126 | issue = 1–2 | pages = 37–46 | year = 1999 | pmid = 9872132 | doi = 10.1016/S0378-5955(98)00148-8 | s2cid = 20953392 }} * {{cite journal | vauthors = Winston JT, Strack P, Beer-Romero P, Chu CY, Elledge SJ, Harper JW | title = The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | journal = Genes Dev. | volume = 13 | issue = 3 | pages = 270–83 | year = 1999 | pmid = 9990852 | pmc = 316433 | doi = 10.1101/gad.13.3.270 }} * {{cite journal | vauthors = Ilyin GP, Rialland M, Glaise D, Guguen-Guillouzo C | title = Identification of a novel Skp2-like mammalian protein containing F-box and leucine-rich repeats. | journal = FEBS Lett. | volume = 459 | issue = 1 | pages = 75–9 | year = 1999 | pmid = 10508920 | doi = 10.1016/S0014-5793(99)01211-9 | s2cid = 22945082 | doi-access = free }} * {{cite journal | vauthors = Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M | title = Identification of a family of human F-box proteins. | journal = Curr. Biol. | volume = 9 | issue = 20 | pages = 1177–9 | year = 1999 | pmid = 10531035 | doi = 10.1016/S0960-9822(00)80020-2 | s2cid = 7467493 | doi-access = free | bibcode = 1999CBio....9.1177C }} * {{cite journal | vauthors = Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW | title = A family of mammalian F-box proteins. | journal = Curr. Biol. | volume = 9 | issue = 20 | pages = 1180–2 | year = 1999 | pmid = 10531037 | doi = 10.1016/S0960-9822(00)80021-4 | s2cid = 14341845 | doi-access = free | bibcode = 1999CBio....9.1180W }} * {{cite journal | vauthors = Suzuki H, Chiba T, Suzuki T, Fujita T, Ikenoue T, Omata M, Furuichi K, Shikama H, Tanaka K | title = Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination. | journal = J. Biol. Chem. | volume = 275 | issue = 4 | pages = 2877–84 | year = 2000 | pmid = 10644755 | doi = 10.1074/jbc.275.4.2877 | doi-access = free }} * {{cite journal | vauthors = Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, Pierce JW, Podust VN, Luo RS, Chau V, Palombella VJ | title = Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha. | journal = Mol. Cell. Biol. | volume = 20 | issue = 7 | pages = 2326–33 | year = 2000 | pmid = 10713156 | pmc = 85397 | doi = 10.1128/MCB.20.7.2326-2333.2000 }} * {{cite journal | vauthors = Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS, Elledge SJ, Harper JW, Pagano M, Pavletich NP | title = Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | journal = Nature | volume = 408 | issue = 6810 | pages = 381–6 | year = 2000 | pmid = 11099048 | doi = 10.1038/35042620 | bibcode = 2000Natur.408..381S | s2cid = 4300503 }} * {{cite journal | vauthors = Lyapina S, Cope G, Shevchenko A, Serino G, Tsuge T, Zhou C, Wolf DA, Wei N, Shevchenko A, Deshaies RJ | title = Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome. | journal = Science | volume = 292 | issue = 5520 | pages = 1382–5 | year = 2001 | pmid = 11337588 | doi = 10.1126/science.1059780 | bibcode = 2001Sci...292.1382L | s2cid = 14224920 }} * {{cite journal | vauthors = Fukuchi M, Imamura T, Chiba T, Ebisawa T, Kawabata M, Tanaka K, Miyazono K | title = Ligand-dependent degradation of Smad3 by a ubiquitin ligase complex of ROC1 and associated proteins. | journal = Mol. Biol. Cell | volume = 12 | issue = 5 | pages = 1431–43 | year = 2001 | pmid = 11359933 | pmc = 34595 | doi = 10.1091/mbc.12.5.1431 }} {{Refend}}

== External links == * {{PDBe-KB2|P63208|S-phase kinase-associated protein 1}}

{{PDB Gallery|geneid=6500}}