{{Short description|Database of DNA polymerases}} {{infobox biodatabase |title = Polbase |logo = |description = Comprehensive database covering various aspects of DNA polymerases |scope = Biology, biochemistry and structure of DNA polymerases |organism = [http://polbase.neb.com/organisms Polbase organisms list] |center = New England Biolabs |laboratory = |author = Brad Langhorst and Nicole Nichols |pmid = 21993301 |released = |standard = |format = |url = [http://polbase.neb.com/ Polbase] |download = |webservice = |sql = |sparql = |webapp = |standalone = |license = |versioning = |frequency = |curation = |bookmark = |version= }}
'''Polbase''' ('''DNA Polymerase Database''') is an open repository of DNA polymerase information.<ref>{{cite journal |doi=10.1093/nar/gkr847 |title=Polbase: A repository of biochemical, genetic and structural information about DNA polymerases |year=2011 |last1=Langhorst |first1=Bradley W. |last2=Jack |first2=William E. |last3=Reha-Krantz |first3=Linda |last4=Nichols |first4=Nicole M. |journal=Nucleic Acids Research |pmid=21993301 |volume=40 |issue=Database issue |pmc=3245023 |pages=D381–7}}</ref> Polbase captures information from published research on polymerase activity, and presents it in context with related work. Polbase is a comprehensive repository<ref>{{Cite book |last=Maloy |first=Stanley |url=https://www.google.com/books/edition/Brenner_s_Encyclopedia_of_Genetics/4cj64BhrnjcC?hl=en&gbpv=1&dq=polbase.neb.com&pg=RA1-PA378&printsec=frontcover |title=Brenner's Encyclopedia of Genetics |last2=Hughes |first2=Kelly |date=2013-03-03 |publisher=Academic Press |isbn=978-0-08-096156-9 |language=en}}</ref> from the 1950s to the present and includes hundreds of polymerases and their related mutants. Polbase's collaborative model allows polymerase investigators to complete, correct and validate Polbase's representation of their work.
== Content == Polbase features a listing of known polymerases categorized by organism, polymerase family, and selected properties. Each indexed polymerase has its own snapshot page containing links to all its information in the database. All results in Polbase are stored with the relevant experimental details to put them into context. If structure information is available, Polbase links to the polymerase's Protein Data Bank (PDB) entry. All information gathered in Polbase is linked to the original publication where it was reported.
== Information sources == Polbase draws information from a variety of sources including PubMed, PDB, and directly from polymerase investigators.
== Interconnections == Polbase is connected with various other databases. These include: * The Protein Data Bank * [http://www.ebi.ac.uk/ European Bioinformatics Institute] * [http://expasy.org/ ExPASy Bioinformatics Resource Portal] * UniProt * BRENDA * PubMed * Various Scientific Journals
== History == Polbase began in March 2009 with a grant from the NIH's SBIR program<ref>[https://reporter.nih.gov/project-details/projectreporter.nih.gov/project_info_description.cfm?icde=0&aid=7936845 NIH SBIR Grant ##1R44GM087021]</ref> and was first presented to the public at MIT's DNA and Mutagenesis Meeting <ref>
[http://cehs.mit.edu/Events0910.html Polbase at DRAM] {{webarchive|url=https://web.archive.org/web/20120328130511/http://cehs.mit.edu/Events0910.html |date=2012-03-28 }}</ref> In March 2010 Polbase was presented to a larger audience at the Evolving Polymerases 2010 Conference.<ref>[http://www.polymerase2010.ch/index.php?option=com_content&view=article&id=56&Itemid=56 Evolving Polymerases Conference 2010] {{webarchive|url=https://web.archive.org/web/20120328125418/http://www.polymerase2010.ch/index.php?option=com_content&view=article&id=56&Itemid=56 |date=2012-03-28 }}</ref> Polbase was also presented in more technical detail at the Rocky 2010 ISMB Conference.<ref>[http://www.iscb.org/cms_addon/conferences/rocky10/abstracts/langhorst.htm Abstract from the ISMB Rocky 2010 Conferences]</ref><ref>[http://www.iscb.org/cms_addon/conferences/rocky10/pdf/Rocky10-program-ALL2.pdf Rocky 2010 conference schedule]</ref> Polbase is described in more detail in the 2012 Nucleic Acids Research Database Issue.
Polbase was built at New England Biolabs by Brad Langhorst and Nicole Nichols with the help of founding collaborators Linda Reha-Krantz, Bill Jack, Cathy Joyce, Stu Linn, Stefan Sarafianos, Sam Wilson, and Roger Woodgate.
== References == {{Reflist}}
== External links == * [http://polbase.neb.com The DNA Polymerase Database (Polbase)]
<!--- Categories ---> Category:Enzyme databases Category:DNA replication