{{Short description|Protein-coding gene in humans}} {{Infobox_gene}} '''Mediator of RNA polymerase II transcription subunit 14''' is an enzyme that in humans is encoded by the ''MED14'' gene.<ref name="pmid9989412">{{cite journal |vauthors=Ryu S, Zhou S, Ladurner AG, Tjian R | title = The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1 | journal = Nature | volume = 397 | issue = 6718 | pages = 446–50 |date=Feb 1999 | pmid = 9989412 | doi = 10.1038/17141 | bibcode =1999Natur.397..446R | s2cid = 4405569 | hdl = 11858/00-001M-0000-0019-A36A-8 | hdl-access = free }}</ref><ref name="pmid9598311">{{cite journal |vauthors=Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K | title = Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method | journal = Genomics | volume = 49 | issue = 2 | pages = 237–46 |date=Aug 1998 | pmid = 9598311 | doi = 10.1006/geno.1998.5246 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: CRSP2 cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa| url = https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=9282}}</ref>
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.<ref name="entrez" />
==Interactions== MED14 has been shown to interact with PPARGC1A,<ref name=pmid14636573>{{cite journal |last=Wallberg |first=Annika E |author2=Yamamura Soichiro |author3=Malik Sohail |author4=Spiegelman Bruce M |author5=Roeder Robert G |date=Nov 2003 |title=Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha |journal=Mol. Cell |volume=12 |issue=5 |pages=1137–49 | issn = 1097-2765| pmid = 14636573 |doi=10.1016/S1097-2765(03)00391-5 |doi-access=free }}</ref> Estrogen receptor alpha,<ref name=pmid11867769>{{cite journal |last=Kang |first=Yun Kyoung |author2=Guermah Mohamed |author3=Yuan Chao-Xing |author4=Roeder Robert G |date=Mar 2002 |title=The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue=5 |pages=2642–7 | issn = 0027-8424| pmid = 11867769 |doi = 10.1073/pnas.261715899 | bibcode =2002PNAS...99.2642K |pmc=122401 |doi-access=free }}</ref> STAT2,<ref name=pmid12509459>{{cite journal |last=Lau |first=Joe F |author2=Nusinzon Inna |author3=Burakov Darya |author4=Freedman Leonard P |author5=Horvath Curt M |date=Jan 2003 |title=Role of metazoan mediator proteins in interferon-responsive transcription |journal=Mol. Cell. Biol. |volume=23 |issue=2 |pages=620–8 | issn = 0270-7306| pmid = 12509459 |doi=10.1128/MCB.23.2.620-628.2003 |pmc=151539 }}</ref> Cyclin-dependent kinase 8,<ref name=pmid11416138>{{cite journal |last=Wang |first=G |author2=Cantin G T |author3=Stevens J L |author4=Berk A J |date=Jul 2001 |title=Characterization of mediator complexes from HeLa cell nuclear extract |journal=Mol. Cell. Biol. |volume=21 |issue=14 |pages=4604–13 | issn = 0270-7306| pmid = 11416138 |doi = 10.1128/MCB.21.14.4604-4613.2001 |pmc=87123 }}</ref><ref name=pmid10198638>{{cite journal |last=Ito |first=M |author2=Yuan C X |author3=Malik S |author4=Gu W |author5=Fondell J D |author6=Yamamura S |author7=Fu Z Y |author8=Zhang X |author9=Qin J |author10=Roeder R G |date=Mar 1999 |title=Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators |journal=Mol. Cell |volume=3 |issue=3 |pages=361–70 | issn = 1097-2765| pmid = 10198638 |doi=10.1016/S1097-2765(00)80463-3 |doi-access=free }}</ref> Glucocorticoid receptor<ref name=pmid10508170>{{cite journal |last=Hittelman |first=A B |author2=Burakov D |author3=Iñiguez-Lluhí J A |author4=Freedman L P |author5=Garabedian M J |date=Oct 1999 |title=Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins |journal=EMBO J. |volume=18 |issue=19 |pages=5380–8 | issn = 0261-4189| pmid = 10508170 |doi = 10.1093/emboj/18.19.5380 |pmc=1171607 }}</ref> and Hepatocyte nuclear factor 4 alpha.<ref name=pmid12089346>{{cite journal |last=Maeda |first=Yutaka |author2=Rachez Christophe |author3=Hawel Leo |author4=Byus Craig V |author5=Freedman Leonard P |author6=Sladek Frances M |date=Jul 2002 |title=Polyamines modulate the interaction between nuclear receptors and vitamin D receptor-interacting protein 205 |journal=Mol. Endocrinol. |volume=16 |issue=7 |pages=1502–10 | issn = 0888-8809| pmid = 12089346 |doi=10.1210/mend.16.7.0883 |doi-access=free }}</ref><ref name=pmid12101254>{{cite journal |last=Malik |first=Sohail |author2=Wallberg Annika E |author3=Kang Yun Kyoung |author4=Roeder Robert G |date=Aug 2002 |title=TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4 |journal=Mol. Cell. Biol. |volume=22 |issue=15 |pages=5626–37 | issn = 0270-7306| pmid = 12101254 |doi=10.1128/MCB.22.15.5626-5637.2002 |pmc=133960 }}</ref>
==References== {{reflist}}
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U.S.A. |volume=93 |issue= 16 |pages= 8329–33 |year= 1996 |pmid= 8710870 |doi=10.1073/pnas.93.16.8329 | pmc=38670 |bibcode=1996PNAS...93.8329F |doi-access=free }} *{{cite journal | author=Yu W |title=Large-scale concatenation cDNA sequencing |journal=Genome Res. |volume=7 |issue= 4 |pages= 353–8 |year= 1997 |pmid= 9110174 |doi= 10.1101/gr.7.4.353| pmc=139146 |name-list-style=vanc| author2=Andersson B | author3=Worley KC | display-authors=3 | last4=Muzny | first4=DM | last5=Ding | first5=Y | last6=Liu | first6=W | last7=Ricafrente | first7=JY | last8=Wentland | first8=MA | last9=Lennon | first9=G }} *{{cite journal | author=Sun X |title=NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription |journal=Mol. Cell |volume=2 |issue= 2 |pages= 213–22 |year= 1998 |pmid= 9734358 |doi=10.1016/S1097-2765(00)80131-8 |name-list-style=vanc| author2=Zhang Y | author3=Cho H | display-authors=3 | last4=Rickert | first4=Paula | last5=Lees | first5=Emma | last6=Lane | first6=William | last7=Reinberg | first7=Danny | doi-access=free }} *{{cite journal | author=Gu W |title=A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation |journal=Mol. Cell |volume=3 |issue= 1 |pages= 97–108 |year= 1999 |pmid= 10024883 |doi=10.1016/S1097-2765(00)80178-1 |name-list-style=vanc| author2=Malik S | author3=Ito M | display-authors=3 | last4=Yuan | first4=Chao-Xing | last5=Fondell | first5=Joseph D | last6=Zhang | first6=Xiaolong | last7=Martinez | first7=Ernest | last8=Qin | first8=Jun | last9=Roeder | first9=Robert G | doi-access=free }} *{{cite journal | author=Ito M |title=Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators |journal=Mol. Cell |volume=3 |issue= 3 |pages= 361–70 |year= 1999 |pmid= 10198638 |doi=10.1016/S1097-2765(00)80463-3 |name-list-style=vanc| author2=Yuan CX | author3=Malik S | display-authors=3 | last4=Gu | first4=Wei | last5=Fondell | first5=Joseph D | last6=Yamamura | first6=Soichiro | last7=Fu | first7=Zheng-Yuan | last8=Zhang | first8=Xiaolong | last9=Qin | first9=Jun | doi-access=free }} *{{cite journal | author=Rachez C |title=Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex |journal=Nature |volume=398 |issue= 6730 |pages= 824–8 |year= 1999 |pmid= 10235266 |doi= 10.1038/19783 |name-list-style=vanc| author2=Lemon BD | author3=Suldan Z | display-authors=3 | last4=Suldan | first4=Zalman | last5=Bromleigh | first5=Virginia | last6=Gamble | first6=Matthew | last7=Näär | first7=Anders M. | last8=Erdjument-Bromage | first8=Hediye | last9=Tempst | first9=Paul |bibcode=1999Natur.398..824R|s2cid=204992765 }} *{{cite journal | author=Näär AM |title=Composite co-activator ARC mediates chromatin-directed transcriptional activation |journal=Nature |volume=398 |issue= 6730 |pages= 828–32 |year= 1999 |pmid= 10235267 |doi= 10.1038/19789 |name-list-style=vanc| author2=Beaurang PA | author3=Zhou S | display-authors=3 | last4=Zhou | first4=Sharleen | last5=Abraham | first5=Shaji | last6=Solomon | first6=William |bibcode=1999Natur.398..828N|s2cid=23646963 }} *{{cite journal | author=Hittelman AB |title=Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins |journal=EMBO J. |volume=18 |issue= 19 |pages= 5380–8 |year= 1999 |pmid= 10508170 |doi= 10.1093/emboj/18.19.5380 | pmc=1171607 |name-list-style=vanc| author2=Burakov D | author3=Iñiguez-Lluhí JA | display-authors=3 | last4=Freedman | first4=LP | last5=Garabedian | first5=MJ }} *{{cite journal | author=Ge K |title=Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis |journal=Nature |volume=417 |issue= 6888 |pages= 563–7 |year= 2002 |pmid= 12037571 |doi= 10.1038/417563a |name-list-style=vanc| author2=Guermah M | author3=Yuan CX | display-authors=3 | last4=Ito | first4=Mitsuhiro | last5=Wallberg | first5=Annika E. | last6=Spiegelman | first6=Bruce M. | last7=Roeder | first7=Robert G. |bibcode=2002Natur.417..563G|s2cid=4432077 }} *{{cite journal |vauthors=Malik S, Wallberg AE, Kang YK, Roeder RG |title=TRAP/SMCC/mediator-dependent transcriptional activation from DNA and chromatin templates by orphan nuclear receptor hepatocyte nuclear factor 4 |journal=Mol. Cell. Biol. |volume=22 |issue= 15 |pages= 5626–37 |year= 2002 |pmid= 12101254 |doi=10.1128/MCB.22.15.5626-5637.2002 | pmc=133960 }} *{{cite journal |vauthors=Wang Q, Sharma D, Ren Y, Fondell JD |title=A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression |journal=J. Biol. Chem. |volume=277 |issue= 45 |pages= 42852–8 |year= 2003 |pmid= 12218053 |doi= 10.1074/jbc.M206061200 |doi-access= free }} *{{cite journal | author=Strausberg RL |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences |journal=Proc. Natl. Acad. Sci. 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