{{Multiple issues| {{COI|date=June 2011}} {{primary sources|date=June 2011}} }}
The term kodecyte is used to describe cells with detectable Function-Spacer-Lipid (FSL) constructs,<ref name="Heathcote2010"/><ref name="Henry2009"/><ref name="Oliver2011a"/><ref name="Oliver2011b"/> and in concert, the term '''kodevirion''' (pronounced co-da-virion), is used to describe virions with detectable FSL constructs.<ref name="Hadac2011"/><ref name="Blake2011"/>
The method for labeling virions with FSL constructs is simple, non covalent and only involves incubation of the virion with the FSL construct in saline for a few hours – nothing further is required.<ref name="Hadac2011" /><ref name="Blake2011" /> The FSL construct will spontaneously, stably and quantitatively incorporate into the virion membrane. Virions have been labelled with fluorescent (FSL-FLRO4) and radioactive iodine (FSL-125I). FSL-FLRO4 could be shown to label virions in a dose dependent manner and could be visualized by flow cytometry either directly, or indirectly if the virion had bound to the cell or fused with the cell membrane.<ref name="Blake2011" /> FSLs do not appear to significantly affect the virions infectivity or their ability to bind target cells, probably because they integrate into the membrane without exposing the virion to chemical agents or covalent modification{{Citation needed|date=June 2011}}.
==See also== * Function-Spacer-Lipid construct (Kode™ Technology) * Kodecyte
==References== {{reflist| refs= <ref name="Heathcote2010">{{cite journal|vauthors=Heathcote D, Carroll T, Wang JJ, Flower R, Rodionov I, Tuzikov A, Bovin N, Henry S |title=Novel antibody screening cells, MUT+Mur kodecytes, created by attaching peptides onto erythrocytes|journal= Transfusion|doi=10.1111/j.1537-2995.2009.02480.x|volume=50|issue=3|pages=635–641|year=2010|pmid=19912581|s2cid=20952307 }}</ref> <ref name="Henry2009">{{cite journal|author=Henry S|title=Modification of red blood cells for laboratory quality control use|journal=Current Opinion in Hematology|doi=10.1097/MOH.0b013e328331257e|volume=16|issue=6|pages=467–472|year=2009|pmid=19680123|s2cid=37416831 }}</ref> <ref name="Oliver2011a">{{cite journal|vauthors=Oliver C, Blake D, Henry S |title=In vivo neutralization of anti-A and successful transfusion of A antigen incompatible red cells in an animal model|journal=Transfusion |doi=10.1111/j.1537-2995.2011.03184.x|year=2011|pmid=21599675|volume=51|issue=12|pages=2664–75|s2cid=205724219 }}</ref> ,<ref name="Oliver2011b">{{cite journal|vauthors=Oliver C, Blake D, Henry S|journal=Transfusion |volume=51 |issue=8 |pages=1723–1730 |doi=10.1111/j.1537-2995.2010.03034.x|year=2011|pmid=21303367|title=Modeling transfusion reactions and predicting in vivo cell survival with kodecytes |s2cid=24736518 }}</ref> and in concert, the term '''kodevirion''' (pronounced co-da-virion), is used to describe virions with detectable Function-Spacer-Lipid (FSL) constructs. <ref name="Hadac2011">{{cite journal|vauthors=Hadac EM, Federspiel MJ, Chernyy E, Tuzikov A, Korchagina E, Bovin NV, Russell S, Henry SM |title=Fluorescein and radiolabeled Function-Spacer-Lipid constructs allow for simple in vitro and in vivo bioimaging of enveloped virions|journal= Journal of Virological Methods|volume=176|issue=1–2|pages=78–84|year=2011|doi=10.1016/j.jviromet.2011.06.005|pmid=21703308 }}</ref> <ref name="Blake2011">{{cite journal|vauthors=Blake DA, Bovin NV, Henry SM |title=FSL Constructs: A simple method for modifying cell/virion surfaces with a range of biological markers without affecting their viability<!--|doi=10.3791/3289-->|issue=54 |article-number=e3289|journal=Journal of Visualized Experiments|year=2011|doi=10.3791/3289|pmid=21847082 |pmc=3211133}}</ref> }}
Category:Biochemistry Category:Biotechnology Category:Laboratory techniques Category:Molecular biology techniques