In the fields of toxicology and pathology, the '''Irwin screen''' is utilised to determine whether the subject(s) show adverse effects from a course of pharmaceutical treatment or environmental pollution. It is an observational methodology.<ref name="moser">{{cite journal |doi=10.1177/0192623310385255|pmid=21075917|title=Functional Assays for Neurotoxicity Testing|journal=Toxicologic Pathology|volume=39|issue=1|pages=36–45|year=2011|last1=Moser|first1=Virginia C.|doi-access=free}}</ref>
==History== Mice were first used systematically to determine a drug's central nervous system side effects by S. Irwin in 1962 and then again in 1968.<ref name=moser/> Its use in the pharmaceutical industry has become ingrained since then, as below.
The National Academy of Sciences issued in 1975 a position paper on the "Principles for Evaluating Chemicals in the Environment." This paper influenced government and academic circles, and was adopted by e.g. Brimblecombe for his study of atmospheric arsenic levels.<ref name="comb">{{cite journal |pmid=552598|year=1979|last1=Brimblecombe|first1=P.|author1-link=Peter Brimblecombe|title=Atmospheric arsenic|journal=Nature|volume=280|issue=5718|pages=104–5|doi=10.1038/280104a0|bibcode=1979Natur.280..104B|s2cid=29678462|doi-access=free}}</ref> The critical review in 1982 by Mitchell and Tilson,<ref name="mt">{{cite journal |pmid=6756792|year=1982|last1=Mitchell|first1=C. L.|title=Behavioral toxicology in risk assessment: Problems and research needs|journal=Critical Reviews in Toxicology|volume=10|issue=4|pages=265–74|last2=Tilson|first2=H. A.|doi=10.3109/10408448209003367}}</ref> caused the US EPA to develop guidelines for several behavioural tests including a test series based on the Irwin Screen, named the Functional Observational Battery (FOB) by Sette in 1989.<ref name="sette">{{cite journal |pmid=2728014|year=1989|last1=Sette|first1=W. F.|title=Adoption of new guidelines and data requirements for more extensive neurotoxicity testing under FIFRA|journal=Toxicology and Industrial Health|volume=5|issue=2|pages=181–94|doi=10.1177/074823378900500204|bibcode=1989ToxIH...5..181S |s2cid=23692531}}</ref> In 1998, the FOB was published in the late 1990s as EPA Human Health 870 Series Test Guidelines,<ref name=moser/><ref name="epa1">{{cite web |url=https://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=P100G6U5.TXT|title = Document Display (PURL) | NSCEP | US EPA}}</ref><ref name="epa2">{{cite web |url=https://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=P100IRWO.TXT|title = Document Display (PURL) | NSCEP | US EPA}}</ref> and in praxis the Irwin screen and the FOB "overlap and to some extent are interchangeable."<ref name=moser/>
The American batteries were harmonised with the OECD's from the same era.<ref name="oecd1">{{Cite web|url=https://www.oecd.org/env/test-no-407-repeated-dose-28-day-oral-toxicity-study-in-rodents-9789264070684-en.htm|title=Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents - en - OECD|date=15 October 2008 }}</ref><ref>{{cite web |title=Organization for Economic Cooperation and Development (OECD) (1997). Guideline 424: Neurotoxicity Study in Rodents |publisher=OECD}}</ref><ref name="jpo">{{Cite web|url=http://www.nihs.go.jp/hse/chem-info/oecd/160603_sec4.pdf|title=OECD Guidelines for the Testing of Chemicals, Section 4 Health Effects}}</ref><ref name="oecdg">{{cite book |title=OECD Guidelines for the Testing of Chemicals / OECD Series on Testing and Assessment Guidance Notes for Analysis and Evaluation of Chronic Toxicity and Carcinogenicity Studies |url=https://books.google.com/books?id=2NHLUhDP2R0C|isbn = 9789264078499|date = 2002-12-12| publisher=OECD }}</ref> Similar tests on food chemicals were recommended by the FDA in their '''Red Book'''.<ref name="redbook">{{cite journal |pmid=9086506|year=1996|last1=Sobotka|first1=T. J.|title=Food and Drug Administration Proposed Guidelines for Neurotoxicological Testing of Food Chemicals|journal=Neurotoxicology|volume=17|issue=3–4|pages=825–36|last2=Ekelman|first2=K. B.|last3=Slikker Jr|first3=W.|last4=Raffaele|first4=K.|last5=Hattan|first5=D. G.}}</ref> Behavioural test batteries are now required for new drugs by the S7A group of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH).{{cn|date=October 2023}}
==Interchangeability with FOB== The Irwin screen was as of 2010 in the pharmaceutical industry almost exclusively used with lab mice, whereas the FOB, or some modification thereof, was used with lab rats and other nonrodent species, such as rabbits, dogs, guinea pigs and nonhuman primates.<ref name=moser/>
==Sample checklist== A sample Irwin screen<ref>{{Cite patent|country=US|number=6664397|title=Use of (+)mefloquine for the treatment of malaria|pubdate=2003-12-16|assign=Vernalis Research Ltd. |inventor1-last=Fletcher|inventor1-first=Allan|inventor2-last=Shepherd|inventor2-first=Robin}}</ref> includes overt behavior observations and autonomic observations:
'''Overt Behavior''' * increased activity * decreased activity * sedation * tremor * convulsions ** myoclonic ** tonic/clonic * straub tail * stereotypy * increased exploration * decreased exploration * ataxia * weakness * catalepsy * pain threshold (tail pinch) * loss of righting reflex * writhing (i.p. administration)
'''Autonomic Observations''' * piloerection * diarrhoea * exophthalmos * salivation * vasoconstriction * vasodilatation * cyanosis * lacrymation * ptosis * miosis * mydriasis * hypothermia
==References== {{reflist}}
Category:Toxicology Category:Environmental toxicology Category:Pathology Category:Pharmacology Category:Neurology Category:Laboratory mice Category:Clinical research Category:Pharmaceuticals policy Category:Drug safety Category:Life sciences industry