{{Short description|Fixative solution}} '''Carnoy's solution''' is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride.<ref>{{cite web |accessdate=13 Jan 2009 |title=MSDS :: Carnoy's Solution (Fixative) |url=http://www.sciencestuff.com/msds/C1466.html |url-status=dead |archiveurl=https://web.archive.org/web/20090327061520/http://www.sciencestuff.com/msds/C1466.html |archivedate=2009-03-27 }}</ref><ref>{{cite journal |author=Carnoy J. B. |title=Appendice Les Globule Polaires de L'Ascaris Clavata |journal=La Cellule RECUEIL DE CYTOLOGIE ET d'HISTOLOGIE GÉNÉRALE |volume=3 |pages=276 |date=1887 |url=https://www.biodiversitylibrary.org/page/6937354}}</ref>
'''Carnoy's solution''' is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).<ref>{{cite journal | doi = 10.3109/10520296209114563 | pmid=13921436 | volume=37 | title=Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration | year=1962 | journal=Stain Technology | pages=17–20 | vauthors=Tjio JH, Whang J}}</ref><ref>{{cite journal | doi = 10.2307/1538691 | volume=104 | issue=1 | year=1953 | journal=The Biological Bulletin | pages=57–67 | vauthors=Mazia D, Brewer PA, Alfert MA| jstor=1538691 | title=The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue | url=https://www.biodiversitylibrary.org/part/32681 }}</ref><ref>{{cite journal | doi = 10.1007/s11230-009-9198-2 | pmid=19731093 | volume=74 | issue=2 | title=Chromosome numbers for three species of medicinal leeches (Hirudo spp.) | year=2009 | journal=Systematic Parasitology | pages=95–102 | vauthors=Utevsky S, Kovalenko N, Doroshenko K, Petrauskienė L, Klymenko V| s2cid=7947757 }}</ref>
The invention of Carnoy's solution is attributed to Jean-Baptiste Carnoy, a pioneering 19th century cytologist.<ref>{{Citation |last1=Ireland |first1=Robert |title=Carnoy's solution |date=2020-04-23 |url=https://www.oxfordreference.com/display/10.1093/acref/9780191828621.001.0001/acref-9780191828621-e-5108;jsessionid=EA246F7FF0D191C9A7457E5A9B9974B3 |work=A Dictionary of Dentistry |editor-last=Yeung |editor-first=Chuen Albert |access-date=2023-04-10 |publisher=Oxford University Press |language=en |doi=10.1093/acref/9780191828621.001.0001 |isbn=978-0-19-182862-1 |last2=Yeung |first2=Chuen Albert YeungChuen Albert|url-access=subscription }}</ref>
==Uses== Some of the uses of Carnoy's solution are: *Enhancing lymph node detection during dissection of cadavers.<ref name="pmid19076686">{{cite journal |vauthors=Luz DA, Ribeiro U, Chassot C, Collet E, Silva FS, Cecconello I, Corbett CE |title=Carnoy's solution enhances lymph node detection: an anatomical dissection study in cadavers |journal=Histopathology |volume=53 |issue=6 |pages=740–2 |date=December 2008 |pmid=19076686 |doi=10.1111/j.1365-2559.2008.03148.x |s2cid=19362456 |issn= }}</ref> *Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.<ref name="pmid12868075">{{cite journal |vauthors=Yoneyama M, Kitayama T, Taniura H, Yoneda Y |title=Immersion fixation with Carnoy solution for conventional immunohistochemical detection of particular N-methyl-D-aspartate receptor subunits in murine hippocampus |journal=J. Neurosci. Res. |volume=73 |issue=3 |pages=416–26 |date=August 2003 |pmid=12868075 |doi=10.1002/jnr.10622 |s2cid=40884078 }}</ref> *Applied directly following enucleation for the treatment of odontogenic keratocysts.<ref name="pmid18353202">{{cite journal |vauthors=Madras J, Lapointe H |title=Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour |journal=J Can Dent Assoc |volume=74 |issue=2 |pages=165–165h |date=March 2008 |pmid=18353202 |url=http://www.cda-adc.ca/jcda/vol-74/issue-2/165.html }}</ref><ref>{{cite web |url=http://www.thejcdp.com/issue002/oda/04oda.htm |title=Odontogenic Keratocyst: The Northwestern USA Experience |accessdate=14 Jan 2009 |url-status=dead |archiveurl=https://web.archive.org/web/20090106171206/http://www.thejcdp.com/issue002/oda/04oda.htm |archivedate=2009-01-06 }}</ref><ref>{{cite web |url=http://iadr.confex.com/iadr/2006Orld/techprogram/abstract_76521.htm |accessdate=14 Jan 2009 |title=Use of Carnoy's Solution in management of odontogenic keratocysts |url-status=dead |archiveurl=https://web.archive.org/web/20090517020923/http://iadr.confex.com/iadr/2006Orld/techprogram/abstract_76521.htm |archivedate=2009-05-17 }}</ref> *Direct application following enucleation (Cuba) for certain kinds of unicystic ameloblastomas.<ref name="pmid15290793">{{cite journal |vauthors=Lee PK, Samman N, Ng IO |title=Unicystic ameloblastoma--use of Carnoy's solution after enucleation |journal=Int J Oral Maxillofac Surg |volume=33 |issue=3 |pages=263–7 |date=April 2004 |pmid=15290793 |doi=10.1006/ijom.2003.0496 }}</ref> This appears to decrease the likelihood of recurrence over enucleation alone.<ref name="pmid16782308">{{cite journal |vauthors=Lau SL, Samman N |title=Recurrence related to treatment modalities of unicystic ameloblastoma: a systematic review |journal=Int J Oral Maxillofac Surg |volume=35 |issue=8 |pages=681–90 |date=August 2006 |pmid=16782308 |doi=10.1016/j.ijom.2006.02.016 }}</ref> Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.<ref name="isbn0-86715-390-3">{{cite book |author=Marx, Robert E. |author2=Stern, Diane |title=Oral and maxillofacial pathology: a rationale for diagnosis and treatment |url=https://archive.org/details/oralmaxillofacia00marx |url-access=limited |publisher=Quintessence |location=Chicago |year=2003 |page=[https://archive.org/details/oralmaxillofacia00marx/page/n14 684] |isbn=0-86715-390-3 }}</ref> *As a fixative for pap smear samples.<ref name="pmid18499991">{{cite journal |doi=10.1159/000325477 |vauthors=Shamsi M, Abdali K, Montazer NR, Kumar PV, Tabatabaee HR |title=Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears |journal=Acta Cytol. |volume=52 |issue=2 |pages=187–90 |year=2008 |pmid=18499991 |s2cid=3370408 }}</ref> *As a fixative agent for both nuclear and mitochondrial DNA in various tissues.<ref name="pmid16260588">{{cite journal |vauthors=Miething F, Hering S, Hanschke B, Dressler J |title=Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues |journal=J. Histochem. Cytochem. |volume=54 |issue=3 |pages=371–4 |date=March 2006 |pmid=16260588 |doi=10.1369/jhc.5B6726.2005|doi-access=free }}</ref> *As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.<ref>{{cite web |accessdate=25 Oct 2009 |title=Stains File: Carnoy's Fluid |url=http://stainsfile.info/StainsFile/prepare/fix/fixatives/carnoy.htm |archive-url=https://web.archive.org/web/20101215025156/http://stainsfile.info/StainsFile/prepare/fix/fixatives/carnoy.htm |archive-date=15 December 2010 |url-status=dead }}</ref>
==References== {{reflist|2}}
Category:Histology Category:Pathology Category:Laboratory techniques Category:Medical terminology