Epi-isozizaene 5-monooxygenase

{{Short description|Class of enzymes}} {{Infobox enzyme | Name = Epi-isozizaene 5-monooxygenase | EC_number = 1.14.15.39 | CAS_number = 1207718-51-1 | GO_code = | image = | width = | caption = }} '''Epi-isozizaene 5-monooxygenase''' ({{EC number|1.14.15.39}}, ''CYP170A1'') is an enzyme with systematic name ''(+)-epi-isozizaene,NADPH:oxygen oxidoreductase (5-hydroxylating)''.<ref>{{cite journal | vauthors = Zhao B, Lin X, Lei L, Lamb DC, Kelly SL, Waterman MR, Cane DE | title = Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2) | journal = The Journal of Biological Chemistry | volume = 283 | issue = 13 | pages = 8183–9 | date = March 2008 | pmid = 18234666 | pmc = 2276382 | doi = 10.1074/jbc.M710421200 | doi-access = free }}</ref> This enzyme catalyses the following overall chemical reaction:

{{chemrxn|width=65%| {{chemrxn/cpd|qid=Q27122614|caption=(+)-epi-isozizaene }} {{chemrxn/txt|+ 4 reduced<br>ferredoxin }} {{chemrxn/arw||fwd_in={{chem2|2 O2}}|fwd_out={{chem2|3 H2O}} }} {{chemrxn/cpd|qid=Q27122616|caption=albaflavenone }} {{chemrxn/txt|+ 4 oxidised<br>ferredoxin }} }}

The enzyme from ''Streptomyces coelicolor'' is an oxidoreductase which uses molecular oxygen to insert a hydroxy group into (+)-epi-isozizaene and then further oxidises that intermediate to the ketone product. It requires ferredoxin to transfer electrons to the cytochrome P450 active site.<ref>{{KEGG enzyme|1.14.15.39}}</ref>

== References == {{reflist}}

== External links == * {{MeshName|Epi-isozizaene+5-monooxygenase}}

Category:EC 1.14.15