| Verifiedfields | changed |
|---|---|
| Watchedfields | changed |
| Verifiedrevid | 401248657 |
| Reference | [1] |
| Iupacname | Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate |
| Othernames | 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470 |
Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
See also
References
- ^ "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
- ^ Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
- ^ Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006