{{refimprove|date=November 2024}} {{chembox | Name = ADA | ImageFile = ADA (buffer).png | ImageName = Chemical structure of ADA | SystematicName = 2,2′-[(2-amino-2-oxoethyl)azanediyl]diacetic acid | OtherNames = ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid |Section1={{Chembox Identifiers | InChI = 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13) | InChIKey = QZTKDVCDBIDYMD-UHFFFAOYSA-N | SMILES = C(C(=O)N)N(CC(=O)O)CC(=O)O | CASNo = 26239-55-4 | ChEBI = 43960 | DrugBank = DB02810 | ChemSpiderID = 105243 | UNII = 5C4R3O704E | EC_number = 247-530-0 | PubChem = 117765 }} | Section2 = {{Chembox Properties | C=6 | H=10 | N=2 | O=5 | MolarMass = 190.155 g/mol | pKa = 6.6 }} | Section3 = {{Chembox Hazards | MainHazards = Irritant | FlashPt = Non-flammable | GHSSignalWord = Warning | HPhrases = {{H-phrases|302|315|319|335}} | PPhrases = {{P-phrases|261|264|270|271|280|301+312|302+352|304+340|305+351+338|312|321|330|332+313|337+313|362|403+233|405|501}} }} }}
'''ADA''' is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.
ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup>, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na<sup>+</sup>/K<sup>+</sup>-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.<ref>{{cite web| title=ADA| website=Sigma-Aldrich| url=http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a9883pis.pdf}}</ref>
==References== {{reflist}}
Category:Zwitterions Category:Tertiary amines Category:Dicarboxylic acids Category:Buffer solutions