{{chembox | Verifiedfields = changed | Watchedfields = changed | verifiedrevid = 477235210 | ImageFile=ACES_buffer.svg | ImageSize= | Name = ACES | PIN=2-[(2-Amino-2-oxoethyl)amino]ethane-1-sulfonic acid | OtherNames=''N''-(2-Acetamido)-2-aminoethanesulfonic acid |Section1={{Chembox Identifiers | ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}} | ChemSpiderID = 73843 | InChI = 1/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10) | InChIKey = DBXNUXBLKRLWFA-UHFFFAOYAH | SMILES1 = O=S(=O)(O)CCNCC(=O)N | StdInChI_Ref = {{stdinchicite|correct|chemspider}} | StdInChI = 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10) | StdInChIKey_Ref = {{stdinchicite|correct|chemspider}} | StdInChIKey = DBXNUXBLKRLWFA-UHFFFAOYSA-N | CASNo_Ref = {{cascite|correct|CAS}} | CASNo=7365-82-4 | UNII_Ref = {{fdacite|correct|FDA}} | UNII = I5SPL5YVBY | PubChem=81832 | ChEBI_Ref = {{ebicite|correct|EBI}} | ChEBI = 39062 | SMILES=C(CS(=O)(=O)O)NCC(=O)N }} |Section2={{Chembox Properties | Formula = | C=4 | H=10 | N=2 | O=4 | S=1 | MolarMass=182.199 | Appearance= | Density= | MeltingPt= | BoilingPt= | Solubility= }} |Section3={{Chembox Hazards | MainHazards= | FlashPt= | AutoignitionPt = }} }}

'''ACES''' ('''''N''-(2-acetamido)-2-aminoethanesulfonic acid''') is a chemical compound that is one of Good's buffers. It was developed in the 1960s to provide buffer solutions with pH ranging from 6.15-8.35 for use in various applications. With a p''K''<sub>a</sub> of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.<ref>Good, N.E., "Hydrogen ion buffers for biological research." "Biochemistry", 5(2), 467–477.</ref>

==Applications== ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.<ref>Liu, Q., et al., "pK-matched running buffers for gel electrophoresis." "Anal. Biochemistry.", 270(1):112-122.</ref> ACES use in isoelectric focusing of proteins has also been documented.<ref>Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." "Electrophoresis", 9(2):65-73.</ref> Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE system.<ref>Strating, H., and Clarke, A.J., "Differentiation of bacterial autolysins by zymogram analysis." "Anal. Biochem.", 291(1):149-154.</ref> Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.<ref>Tunnicliff, G., and Smith, J.A., "Competitive inhibition of γ-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-ε-ethanesulfonic acid and related buffer." "J. Neurochem.", 36(3):1122-1126.</ref>

==References== <references/>

Category:Buffer solutions