The 2016 Israel Open was a professional tennis tournament played on hard courts. It was the 5th edition of the tournament, which was part of the 2016 ATP Challenger Tour. It took place in Ra'anana, Israel between 28 March and 3 April.
Singles main-draw entrants
Seeds
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| Russia | Evgeny Donskoy | 82 | 1 |
| Israel | Dudi Sela | 84 | 2 |
| Lithuania | Ričardas Berankis | 86 | 3 |
| Slovakia | Lukáš Lacko | 98 | 4 |
| Italy | Thomas Fabbiano | 110 | 5 |
| Russia | Konstantin Kravchuk | 131 | 6 |
| Turkey | Marsel İlhan | 137 | 7 |
| Switzerland | Marco Chiudinelli | 139 | 8 |
- 1 Rankings are as of March 21, 2016
Other entrants
The following players received wildcards into the singles main draw:
- Dekel Bar
- Daniel Cukierman
- Tal Goldengoren
- Edan Leshem
The following players received entry as alternates:
The following players received entry from the qualifying draw:
Champions
Singles
- Evgeny Donskoy def. Ričardas Berankis, 6–4, 6–4
Doubles
- Konstantin Kravchuk / Denys Molchanov def. Jonathan Erlich / Philipp Oswald, 4–6, 7–6(7–1), [10–4]