# Water blue

> Mediated Wiki article. Canonical URL: https://mediated.wiki/source/Water_blue
> Markdown URL: https://mediated.wiki/source/Water_blue.md
> Source: https://en.wikipedia.org/wiki/Water_blue
> Source revision: 1303163989
> License: Creative Commons Attribution-ShareAlike 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/)

{{For|the butterfly with this common name|Cacyreus tespis{{!}}''Cacyreus tespis''}}
{{refimprove|date=June 2013}}
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| ImageFile = Water blue2.png
| ImageSize = 
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|Section1={{Chembox Identifiers
| CASNo_Ref = {{cascite|correct|??}}
| CASNo = 28631-66-5
| ChemSpiderID = 21173243
| EINECS = 249-113-9
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 44D829LK4D
| PubChem = 139030972
| SMILES = [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
| StdInChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
| StdInChIKey = WJHQBPPQVMBLSW-UHFFFAOYSA-L
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|Section2={{Chembox Properties
| Formula = C<sub>32</sub>H<sub>25</sub>N<sub>3</sub>O<sub>9</sub>S<sub>3</sub>Na<sub>2</sub>
| MolarMass = 737.72374 g/mol
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'''Water blue''', also known as '''aniline blue''', '''Acid blue 22''', '''Soluble Blue 3M''', '''Marine Blue V''', or '''C.I. 42755''', is a [chemical compound](/source/chemical_compound) used as a [stain](/source/staining_(biology)) in [histology](/source/histology).  Water supply blue stains [collagen](/source/collagen) blue in [tissue](/source/biological_tissue) sections. It is soluble in water and slightly soluble in [ethanol](/source/ethanol).

Water blue is also available in mixture with [methyl blue](/source/methyl_blue), under the names [Aniline Blue WS](/source/Aniline_Blue_WS), Aniline blue, China blue, or Soluble blue.<ref>{{cite web |year= 1969 |title= H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory |author= Lillie, R. D. |url= https://trove.nla.gov.au/work/21134552?selectedversion=NBD35338 |access-date= February 8, 2019 |website= Trove (National Library of Australia)}}</ref><ref>{{cite web |title= Aniline Blue WS |website= StainsFile |url= http://stainsfile.info/StainsFile/dyes/707.htm }}</ref>

It can be used in the [Mallory's trichrome stain](/source/Mallory's_trichrome_stain) of [connective tissue](/source/connective_tissue) and [Gömöri trichrome stain](/source/G%C3%B6m%C3%B6ri_trichrome_stain) of muscle tissue. It is used in [differential staining](/source/differential_staining).<ref name= "VCRB2012b">{{Citation|title= A simple dual stain for detailed investigations of plant-fungal pathogen interactions |last=Nowicki|first=Marcin|journal= Vegetable Crops Research Bulletin |date=15 May 2013|volume= 77 |pages= 61–74 |publisher=Vegetable Crops Research bulleting, InHort & Versita|doi= 10.2478/v10032-012-0016-z|display-authors=etal|doi-access=free}}</ref>

== See also ==
*[RAL 5021 Water blue](/source/RAL_5021_Water_blue)

== References ==
{{Reflist}}

Category:Triarylmethane dyes
Category:Staining dyes
Category:Benzenesulfonates
Category:Organic sodium salts
Category:Anilines
Category:Acid dyes

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Adapted from the Wikipedia article [Water blue](https://en.wikipedia.org/wiki/Water_blue) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Water_blue?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.