# Solid phase sequencing

> Mediated Wiki article. Canonical URL: https://mediated.wiki/source/Solid_phase_sequencing
> Markdown URL: https://mediated.wiki/source/Solid_phase_sequencing.md
> Source: https://en.wikipedia.org/wiki/Solid_phase_sequencing
> Source revision: 1192012927
> License: Creative Commons Attribution-ShareAlike 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/)

The principle of '''solid phase DNA sequencing''' was described in 1989 based on binding of [biotinylated](/source/Biotin) [DNA](/source/DNA) to [streptavidin](/source/streptavidin)-coated magnetic beads and [elution](/source/elution) of single DNA strands selectively using [alkali](/source/alkali).<ref>{{cite journal |last1=Hultman |first1=T |last2=Ståhl |first2=S |last3=Hornes |first3=E |last4=Uhlén |first4=M |title=Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. |journal=Nucleic Acids Research |date=11 July 1989 |volume=17 |issue=13 |pages=4937–4946 |pmc=318085 |pmid=2668874 |doi=10.1093/nar/17.13.4937}}</ref> The method allowed robotic applications suitable for clinical sequencing, but the magnetic handling has also found frequent use in many molecular applications, including sample handling for DNA diagnostics.<ref>{{cite journal |last1=Uhlen |first1=M. |title=Magnetic separation of DNA |journal=Nature |date=August 1989 |volume=340 |issue=6236 |pages=733–734 |doi=10.1038/340733a0 |pmid=2770876 |bibcode=1989Natur.340..733U |s2cid=4319266 }}</ref> The use of solid phase methods for DNA handling is now frequently used as an integrated part of many of the next generation [DNA sequencing](/source/DNA_sequencing) methods, as well as numerous [molecular diagnostics](/source/molecular_diagnostics) applications.{{fact|date=February 2020}}

==References==
{{reflist}}

Category:DNA sequencing
Category:Biotechnology

---
Adapted from the Wikipedia article [Solid phase sequencing](https://en.wikipedia.org/wiki/Solid_phase_sequencing) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Solid_phase_sequencing?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.