# Simulated fluorescence process algorithm

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The **Simulated Fluorescence Process** (SFP) is a computing [algorithm](/source/Algorithm) used for [scientific visualization](/source/Scientific_visualization) of [3D data](/source/3D_scanning) from, for example, [fluorescence microscopes](/source/Fluorescence_microscope). By [modeling](/source/Computer_simulation) a physical light/matter interaction process, an image can be computed which shows the data as it would have appeared in reality when viewed under these conditions.

## Principle

The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, which also interacts with the object before it finally reaches the eye of the viewer.[1][2]

## See also

- [Computer graphics lighting](/source/Computer_graphics_lighting)
- [Rendering (computer graphics)](/source/Rendering_(computer_graphics))

## References

1. Voort, H. T. M.; Brakenhoff, G. J.; Baarslag, M. W. (February 1989). ["Three-dimensional visualization methods for confocal microscopy"](http://dx.doi.org/10.1111/j.1365-2818.1989.tb00553.x). *Journal of Microscopy*. **153** (2): 123–132. [doi:10.1111/j.1365-2818.1989.tb00553.x](https://doi.org/10.1111/j.1365-2818.1989.tb00553.x). [ISSN 0022-2720](https://www.worldcat.org/issn/0022-2720). [PMID 2709405](https://pubmed.ncbi.nlm.nih.gov/2709405)

1. Noordmans, H.J.; van der Voort, H.T.M.; Smeulders, A.W.M. (2000). ["Spectral volume rendering"](http://dx.doi.org/10.1109/2945.879782). *IEEE Transactions on Visualization and Computer Graphics*. **6** (3): 196–207. [doi:10.1109/2945.879782](https://doi.org/10.1109/2945.879782). [ISSN 1077-2626](https://www.worldcat.org/issn/1077-2626)

## External links

- [Freeware SFP renderer](https://web.archive.org/web/20060928113641/http://support.svi.nl/wiki/FreeSfp)

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Adapted from the Wikipedia article [Simulated fluorescence process algorithm](https://en.wikipedia.org/wiki/Simulated_fluorescence_process_algorithm) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Simulated_fluorescence_process_algorithm?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.