# SSC buffer

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> Markdown URL: https://mediated.wiki/source/SSC_buffer.md
> Source: https://en.wikipedia.org/wiki/SSC_buffer
> Source revision: 1066119490
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In [biochemistry](/source/biochemistry) and [molecular biology](/source/molecular_biology), '''saline-sodium citrate (SSC) buffer''' is used as a hybridization [buffer](/source/buffer_(chemistry)), to control stringency for washing steps in protocols for [Southern blotting](/source/Southern_blotting), [in situ hybridization](/source/in_situ_hybridization), [DNA Microarray](/source/DNA_Microarray) or [Northern blotting](/source/Northern_blotting). 20X SSC may be used to prevent drying of [agarose gel](/source/agarose_gel)s during a vacuum transfer. 

A 20X stock solution consists of 3 [M](/source/molar_(concentration)) [sodium chloride](/source/sodium_chloride) and 300 [m](/source/milli-)M [trisodium citrate](/source/trisodium_citrate) (adjusted to [pH](/source/pH) 7.0 with [HCl](/source/hydrochloric_acid)).

==See also==
*[Southern Blot](/source/Southern_Blot)
*[Northern Blot](/source/Northern_Blot)
*[DNA Microarray](/source/DNA_Microarray)
*[DNA Fingerprinting](/source/DNA_Fingerprinting)

==References==
* [http://www.cellgro.com/shop/files/documents/46_020.pdf Material safety data sheet for 20x SSC buffer, Mediatech, Inc.]
* [http://www.le.ac.uk/bl/phh4/probuffer.htm Molecular Cytogenetics and biology Buffers, University of Leicester]

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Category:Buffer solutions

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Adapted from the Wikipedia article [SSC buffer](https://en.wikipedia.org/wiki/SSC_buffer) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/SSC_buffer?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.