# NS0 cell

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**NS0 cells** are a model [cell line](/source/Cell_line) derived from the nonsecreting murine myeloma used in [biomedical research](/source/Biomedical_research) and commercially in the production of therapeutic proteins.[1] The cell line is a cholesterol-dependent cell line that was generated from a subline of NSI/1 which produced only the light chain but no heavy chain.[2][3]

## Cell line development

Development of murine neoplasms started with work with the [BALB/c](/source/BALB/c) mice to isolate the [IgG1](/source/IgG1) secreting MOPC21 tumor. From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line secreted only light chain and no heavy chain and was renamed NSI/1. Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1.[2][4]

## Uses in biotechnology

As myeloma cells, NSo cells are naturally antibody-producing suspension cells with a lymphoblast morphology.[5] Gene amplification is typically performed using GS-transfected NS0 cells to select for producing cell lines.[6] The GS-NS0 is a heterologous mammalian expression system that allows for the rapid expression of recombinant proteins. Several therapeutic antibody products are produced using the NS0 cell line including [daclizumab](/source/Daclizumab) and [eculizumab](/source/Eculizumab).[7]

## References

1. Barnes, LM; Bentley, CM; Dickson, AJ (February 2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system.". *Cytotechnology*. **32** (2): 109–23. [doi:10.1023/A:1008170710003](https://doi.org/10.1023/A:1008170710003). [PMC 3449689](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449689). [PMID 19002973](https://pubmed.ncbi.nlm.nih.gov/19002973)

1. M. Barnes, Louise; Bentley, Catherine M.; Dickson, Alan J. (2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system". *Cytotechnology*. **32** (2): 109–123. [doi:10.1023/A:1008170710003](https://doi.org/10.1023/A:1008170710003). [PMC 3449689](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449689). [PMID 19002973](https://pubmed.ncbi.nlm.nih.gov/19002973)

1. Sato, JD; Cao, HT; Kayada, Y; Cabot, MC; Sato, GH; Okamoto, T; Welsh, CJ (December 1988). "Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.". *In Vitro Cellular & Developmental Biology*. **24** (12): 1223–8. [doi:10.1007/bf02624194](https://doi.org/10.1007/bf02624194). [PMID 3209588](https://pubmed.ncbi.nlm.nih.gov/3209588). [S2CID 2470410](https://api.semanticscholar.org/CorpusID:2470410)

1. Galfrè, G & Milstein, C (1981). "[1] Preparation of monoclonal antibodies: Strategies and procedures". *Preparation of monoclonal antibodies: strategies and procedures.*. Vol. 73, no. Pt B. Methods in Enzymology. pp. 3–46. [doi:10.1016/0076-6879(81)73054-4](https://doi.org/10.1016/0076-6879(81)73054-4). ISBN 9780121819736. [PMID 7300683](https://pubmed.ncbi.nlm.nih.gov/7300683)

1. ["NS0 Cell Line from murine myeloma"](https://www.sigmaaldrich.com/catalog/product/sigma/cb_85110503?lang=en&region=US). Retrieved 15 July 2018.

1. Barnes, Louise M.; Bentley, Catherine M.; Dickson, Alan J. (20 May 2001). "Characterization of the stability of recombinant protein production in the GS-NS0 expression system". *Biotechnology and Bioengineering*. **73** (4): 261–270. [doi:10.1002/bit.1059](https://doi.org/10.1002/bit.1059). [PMID 11283909](https://pubmed.ncbi.nlm.nih.gov/11283909)

1. Wurm, Florian M (November 2004). "Production of recombinant protein therapeutics in cultivated mammalian cells". *Nature Biotechnology*. **22** (11): 1393–8. [doi:10.1038/nbt1026](https://doi.org/10.1038/nbt1026). [PMID 15529164](https://pubmed.ncbi.nlm.nih.gov/15529164). [S2CID 20428452](https://api.semanticscholar.org/CorpusID:20428452)

## External links

- [Cellosaurus entry for NS0](https://www.cellosaurus.org/CVCL_3940)

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Adapted from the Wikipedia article [NS0 cell](https://en.wikipedia.org/wiki/NS0_cell) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/NS0_cell?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.