# Lithium acetate

> Mediated Wiki article. Canonical URL: https://mediated.wiki/source/Lithium_acetate
> Markdown URL: https://mediated.wiki/source/Lithium_acetate.md
> Source: https://en.wikipedia.org/wiki/Lithium_acetate
> Source revision: 1333254139
> License: Creative Commons Attribution-ShareAlike 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/)

{{Chembox
| Verifiedfields = changed
| Watchedfields  = changed
| verifiedrevid  = 450703117
| ImageFile      = Lithium acetate.svg
| ImageSize      = 150px
| ImageClass     = skin-invert
| ImageFile2     = Lithium acetate.JPG
| PIN            = Lithium acetate
| OtherNames     = 
| Section1       = {{Chembox Identifiers
| CASNo_Ref = {{cascite|correct|CAS}}
| CASNo = 546-89-4
| CASNo_Comment = (anhydrous)
| CASNo2_Ref = {{cascite|correct|CAS}}
| CASNo2 = 6108-17-4
| CASNo2_Comment = (dihydrate)
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = H4278O5FLV
| EINECS = 208-914-3
| PubChem = 	3474584
| ChemSpiderID_Ref = {{chemspidercite|changed|chemspider}}
| ChemSpiderID = 10562
| SMILES = [Li+].CC(=O)[O-]
| InChI = 1/C2H4O2.Li/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
| InChIKey = XIXADJRWDQXREU-REWHXWOFAX
| StdInChI_Ref = {{stdinchicite|changed|chemspider}}
| StdInChI = 1S/C2H4O2.Li/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
| StdInChIKey_Ref = {{stdinchicite|changed|chemspider}}
| StdInChIKey = XIXADJRWDQXREU-UHFFFAOYSA-M
| MeSHName = C488804
| ChEBI_Ref = {{ebicite|changed|EBI}}
| ChEBI = CHEBI:63045
| KEGG_Ref = {{keggcite|changed|kegg}}
| KEGG = D08134
| RTECS = AI545000
 }}
| Section2       = {{Chembox Properties
| Formula = {{chem2|CH3COOLi}}
| Li=1 | C=2 | H=3 | O=2
| Appearance = White crystalline solid
| Density = 1.26 g/cm<sup>3</sup>
| MeltingPtC = 286
| BoilingPt =
| MagSus = &minus;34.0·10<sup>−6</sup> cm<sup>3</sup>/mol 
| Solubility = 45.0 g/100 mL<ref name="hand">
{{Cite book
| last = Lide
| first = David R.
| author-link = 
| year = 1998
| title = Handbook of Chemistry and Physics
| edition = 87
| volume = 
| series =
| publication-place = Boca Raton, FL
| publisher = CRC Press
| isbn = 0-8493-0594-2
| doi =
| oclc =
| pages = 465
| url = 
}}</ref>
 }}
| Section3       = {{Chembox Hazards
| MainHazards = toxic
| NFPA-H = 2
| NFPA-F = 0
| NFPA-R = 0
| ExternalSDS = [https://fscimage.fishersci.com/msds/95125.htm External MSDS]
| FlashPt =
| AutoignitionPt =
| LD50 = 500 mg/kg (oral, mouse)
 }}
| Section9 = {{Chembox Related
| OtherAnions    = [Lithium formate](/source/Lithium_formate)
| OtherCations   = {{ubl|[Sodium acetate](/source/Sodium_acetate)|[Potassium acetate](/source/Potassium_acetate)|[Rubidium acetate](/source/Rubidium_acetate)|[Cesium acetate](/source/Cesium_acetate)}}
}}
}}
'''Lithium acetate''' is a [lithium](/source/lithium) [salt](/source/salt_(chemistry)) of [acetic acid](/source/acetic_acid), with the [chemical formula](/source/chemical_formula) {{chem2|CH3COOLi}}. It is often abbreviated as LiO[Ac](/source/Acetyl_group). It is a white crystalline solid.

== Uses ==

Lithium acetate is used in the laboratory as [buffer](/source/Buffer_solution) for [gel electrophoresis](/source/gel_electrophoresis) of [DNA](/source/DNA) and [RNA](/source/RNA). It has a lower [electrical conductivity](/source/electrical_conductivity) and can be run at higher speeds than can gels made from [TAE buffer](/source/TAE_buffer) (5-30V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or [Southern blot](/source/Southern_blot) analysis, work as expected when using lithium acetate gels.

[Lithium boric acid](/source/LB_buffer) or [sodium boric acid](/source/SB_buffer) are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.

Lithium acetate is also used to permeabilize the cell wall of [yeast](/source/yeast) for use in DNA [transformation](/source/Transformation_(genetics)). It is believed that the beneficial effect of LiOAc is caused by its [chaotropic effect](/source/chaotropic_agent); denaturing DNA, RNA and proteins.<ref>{{cite journal|last=Norcum|first=MT|title=Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents.|journal=The Journal of Biological Chemistry|date=Aug 15, 1991|volume=266|issue=23|pages=15398–405|doi=10.1016/S0021-9258(18)98629-1|pmid=1651330|doi-access=free}}</ref>

== References ==
{{reflist}}
* {{cite journal | doi = 10.1016/j.ab.2004.05.054 | last1 = Brody | first1 = JR | last2 = Kern | first2 = SE | title = History and principles of conductive media for standard DNA electrophoresis. | journal = [Analytical Biochemistry](/source/Analytical_Biochemistry_(journal)) | year = 2004 | volume = 333 | issue = 1 | pages = 1–13 | pmid = 15351274}}

{{Lithium compounds}}
{{clear}}
{{Acetates}}

Category:Acetates
Category:Lithium salts

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Adapted from the Wikipedia article [Lithium acetate](https://en.wikipedia.org/wiki/Lithium_acetate) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Lithium_acetate?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.