# Fluorogenic

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'''Fluorogenic''' describes a property of chemical compounds which are initially not [fluorescent](/source/Fluorescence), but become fluorescent through a chemical reaction, typically through an intermolecular covalent reaction which binds the now fluorescent compound to a target molecule.<ref name="McChesney-Harris Kakodakar Bernstein Stobaugh 2012 pp. 117–132">{{cite journal | last1=McChesney-Harris | first1=L. L. | last2=Kakodakar | first2=S. V. | last3=Bernstein | first3=S. C. | last4=Stobaugh | first4=J. F. | title=Mechanism Based Design of Amine Fluorogenic Derivatization Reagents: Proof of Concept, Physical–Chemical Characterization and Initial Analytical Derivatization Protocols | journal=Chromatographia | publisher=Springer Science and Business Media LLC | volume=76 | issue=3–4 | date=2012-12-22 | issn=0009-5893 | doi=10.1007/s10337-012-2371-9 | pages=117–132| s2cid=95016112 }}</ref> [IUPAC](/source/IUPAC) uses a broader definition of fluorogenic, wherein an enhancement of fluorescence via a chemical reaction is not required, however in contrast to the IUPAC definition common use of fluorogenic does not refer to non-reaction effects like the enhancement of fluorescence from a [fluorophore](/source/fluorophore) being in different solvents.<ref>{{cite journal |title=Fluorogenic |url=https://goldbook.iupac.org/terms/view/FT07379 |website=IUPAC GoldBook |year=2014 |publisher=IUPAC |doi=10.1351/goldbook.FT07379 |access-date=10 March 2023|doi-access=free |url-access=subscription }}</ref> Fluorogenic labeling reagents are often used in [analytical chemistry](/source/analytical_chemistry) procedures, particularly in [HPLC](/source/High-performance_liquid_chromatography) or [CE](/source/capillary_electrophoresis) to derivative target compounds (e.g. labeling the [primary amines](/source/primary_amine) of [polypeptides](/source/polypeptides)), thereby allowing enhanced sensitivity through fluorescence based detection.<ref name="Fanali Haddad Poole Riekkola 2017 p. ">{{cite book | last1=Fanali | first1=Salvatore | last2=Haddad | first2=Paul R. | last3=Poole | first3=Colin | last4=Riekkola | first4=Marja-Liisa | title=Liquid chromatography. Volume 2, Applications | publication-place=Amsterdam, Netherlands | date=2017 | isbn=978-0-12-809344-3 | oclc=992119225 | language=en | page=}}</ref> 

==Examples==
* [OPA](/source/Phthalaldehyde)
* [Fluorescamine](/source/Fluorescamine)
* [FQ](/source/(3-(2-furoyl)-quinoline-2_carboxaldehyde))
* [NBD-F](/source/4-Fluoro-7-nitrobenzofurazan)
* [6-AQC](/source/6-aminoquinolyl-N-hydroxysuccinimidyl_carbamate)
* [Epicocconone](/source/Epicocconone)
* [CBQCA](/source/CBQCA)

==See also==
* [Colorogenic](/source/Colorogenic)

==References==
{{reflist}}

Category:Fluorescence
Category:Analytical chemistry

{{analytical-chem-stub}}

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Adapted from the Wikipedia article [Fluorogenic](https://en.wikipedia.org/wiki/Fluorogenic) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Fluorogenic?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.