# Dische test

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The **Dische test**, or **Dische reaction**, is used to distinguish [DNA](/source/DNA) from [RNA](/source/RNA). It was invented by [Zacharias Dische](/source/Zacharias_Dische).

## Method

Dische's diphenylamine reagent consists of [diphenylamine](/source/Diphenylamine), glacial acetic acid, sulfuric acid, and ethanol.[1]

When heated with DNA, it turns blue in the presence of DNA. A more intense blue color indicates a greater concentration of DNA.

## Mechanism

The acid converts deoxyribose to a molecule that binds with diphenylamine to form a blue substance. The reagent does not interact with RNA, so can be used to distinguish DNA from RNA.[2][3]

## See also

- [Bial's test](/source/Bial's_test)

## References

1. Patterson, J & Mura, C (4 February 2013). "Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples.". *Journal of Visualized Experiments*. '***(72). [doi:10.3791/50225](https://doi.org/10.3791/50225). [PMC 3597041](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597041). [PMID 23407542](https://pubmed.ncbi.nlm.nih.gov/23407542)***

1. ["Nucleic Acids: DNA extraction and Dische's Diphenylamine test (Activity) | Biology OER"](https://openlab.citytech.cuny.edu/bio-oer/chemistry/biologically-important-macromolecules/nucleic-acids/nucleic-acids-dna-extraction-and-disches-diphenylamine-test-activity/). Retrieved 8 March 2023.

1. ["DNA-Dische Diphenylamine Determination"](https://biocyclopedia.com/index/biotechnology_methods/molecular_biology/dna_dische_diphenylamine_determination.php). *biocyclopedia.com*. Retrieved 8 March 2023.

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