# Broth microdilution

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{{Short description|Antimicrobial resistance test}}
thumb|right|A completed broth microdilution test
'''Broth microdilution''' is a method used to test the susceptibility of [microorganisms](/source/microorganisms) to [antibiotics](/source/antibiotics). It is the most commonly used method to perform this test in the [United States](/source/United_States)<ref name=a>{{cite book|last1=Engelkirk|first1=Paul|last2=Duben-Engelkirk|first2=Janet|title=Laboratory Diagnosis of Infectious Diseases|date=2008|publisher=Lippincott Williams & Wilkins|page=168|isbn=9780781797016|url=https://books.google.com/books?id=l56-WMdyqzcC&dq=broth-microdilution+method&pg=PA168|access-date=16 November 2014}}</ref> and [Europe](/source/Europe). <ref>{{Cite web |title=eucast: MIC determination |url=https://www.eucast.org/ast_of_bacteria/mic_determination |access-date=2024-09-26 |website=www.eucast.org}}</ref> 

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==Process==
During testing, multiple [microtiter plate](/source/microtiter_plate)s are filled with a certain broth, according to the needs of target bacteria.<ref name=c>{{cite book|last1=Lorian|first1=Victor|title=Antibiotics in Laboratory Medicine|date=2005|publisher=Lippincott Williams & Wilkins|page=149|isbn=9780781749831|url=https://books.google.com/books?id=HdA4dl8m_T4C&dq=broth-microdilution+method&pg=PA149|access-date=16 November 2014}}</ref> Varying concentrations of the antibiotics and the bacteria to be tested are then added to the plate. The plate is then placed into a non-{{CO2}} [incubator](/source/Incubator_(culture)) and incubated at thirty-seven degrees [Celsius](/source/Celsius) for sixteen to twenty hours. Following the allotted time, the plate is removed and checked for bacterial growth. If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped bacterial expansion.<ref name="a"/>

==Advantages==
The broth microdilution method can be used to test the susceptibility of microorganisms to multiple antibiotics at once.<ref>{{cite book|last1=Lee|first1=Mary|title=Basic Skills in Interpreting Laboratory Data|date=2013|publisher=ASHP|page=723|isbn=9781585283453|edition=5|url=https://books.google.com/books?id=5WW1AAAAQBAJ&dq=broth-microdilution+method&pg=PT723|access-date=16 November 2014}}</ref> Broth microdilution is also highly accurate. The accuracy of its results are comparable to [agar dilution](/source/agar_dilution), the [gold standard](/source/Gold_standard_(test)) of susceptibility testing. Other advantages include the commercial availability of plates, the ease of testing and storing the plates, and the ability for the results of some tests to be read by machines.<ref name="a"/>

==References==
{{Reflist}}

Category:Antimicrobial resistance

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Adapted from the Wikipedia article [Broth microdilution](https://en.wikipedia.org/wiki/Broth_microdilution) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/Broth_microdilution?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.