# BANA test

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The '''BANA test''' (referring to the [enzymatic](/source/enzyme) [breakdown](/source/chemical_breakdown) of ''N''-benzoyl-{{smallcaps|dl}}-arginine-2-napthylamide) is used to determine the [proteolytic activity](/source/proteolysis) of certain oral [anaerobe](/source/anaerobe)s that contribute to [oral malodor](/source/halitosis). Some [bacteria](/source/bacterium), e.g. Prophyromona gingivalis, [Treponema denticola](/source/Treponema_denticola), and [Bacteroides forsythus](/source/Tannerella_forsythia) (Red complex) produce waste products that are quite odiferous, and as a result contribute to bad breath.<ref>{{Cite journal|pmc = 4555797|year = 2015|last1 = Dhalla|first1 = N|title = The detection of BANA micro-organisms in adult periodontitis before and after scaling and root planing by BANA-Enzymatic™ test kit: An in vivo study|journal = Journal of Indian Society of Periodontology|volume = 19|issue = 4|pages = 401–405|last2 = Patil|first2 = S|last3 = Chaubey|first3 = K. K|last4 = Narula|first4 = I. S|pmid = 26392688|doi = 10.4103/0972-124X.154167 | doi-access=free }}</ref>

When a sample of a [patient's](/source/patient) [saliva](/source/saliva) that contains these bacteria is placed within the BANA testing compound, it causes the breakdown of the N-benzoyl enzyme. As a result of this [biodegradation](/source/biodegradation) occurs, the test compound changes color, indicating a positive reaction.<ref>{{cite book|title=Periodontis Revisited |url=https://books.google.com/books?id=NKe1zjbxpvQC&q=placed+within+the+BANA+testing+compound%2C+it+causes+the+breakdown+of+the+N-benzoyl&pg=PA241 |year=2011 |page=242 |isbn=978-93-5025-367-0|last1=Bathla |first1=Shalu | publisher=JP Medical }}</ref>

Uses: Used to identify volatile Sulphur compounds in halitosis patients.

==References==
{{reflist}}

==Further reading==
* {{cite journal |last1=Andrade |first1=José Alexandre de |last2=Feres |first2=Magda |last3=Figueiredo |first3=Luciene Cristina de |last4=Salvador |first4=Sérgio Luiz |last5=Cortelli |first5=Sheila Cavalca |title=The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia |journal=Brazilian Oral Research |date=June 2010 |volume=24 |issue=2 |pages=224–230 |doi=10.1590/S1806-83242010000200016 |pmid=20658043 |doi-access=free }}
* {{cite journal |last1=Loesche |first1=W. J. |last2=Lopatin |first2=D. E. |last3=Giordano |first3=J. |last4=Alcoforado |first4=G. |last5=Hujoel |first5=P. |title=Comparison of the benzoyl-DL-arginine-naphthylamide (BANA) test, DNA probes, and immunological reagents for ability to detect anaerobic periodontal infections due to Porphyromonas gingivalis, Treponema denticola, and Bacteroides forsythus. |journal=Journal of Clinical Microbiology |date=1 February 1992 |volume=30 |issue=2 |pages=427–433 |doi=10.1128/JCM.30.2.427-433.1992 |pmid=1311335 |pmc=265072 |doi-access=free }}

Category:Biodegradation
Category:Metabolism
Category:Oral hygiene

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