# Alsever's solution

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**Alsever's solution** is a [saline](/source/Salinity) liquid used to prevent coagulation of blood.[1][2] It is composed of 2.05% [dextrose](/source/Dextrose), 0.8% [sodium citrate](/source/Sodium_citrate), 0.055% [citric acid](/source/Citric_acid), and 0.42% [sodium chloride](/source/Sodium_chloride). For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution. This solution is used to study *[in vitro](/source/In_vitro)* anti-inflammatory activity of crude drugs by the [human red blood cell stabilization](/source/Human_red_blood_cell_stabilization) method.[3] It is also used to preserve blood cells from other sources.[4][5]

The test was invented in 1941 by the American Hematologist, John Bellows Alsever (1908–1978).[6]

## References

1. Alsever, J. B., & Ainslie, R. B. (1941). A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. NY State J. Med, 41, 126-131.

1. Campbell, D. H.; Garvey, J. S.; Cremer, N. E.; Sussdorf, D. H. (1963). *Methods in Immunology. A Laboratory Text for Instruction and Research*. New York: W. A. Benjamin, Inc. p. 244.

1. Gandhisan R, Thamaraichelvan A, Baburaj. Antiinflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitotherapia 1991; 62: 82-83.

1. Schjerning-Thiesen, Knud (2009). "Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution". *Acta Pathologica et Microbiologica Scandinavica*. **32** (1): 198–203. [doi:10.1111/j.1699-0463.1953.tb00242.x](https://doi.org/10.1111/j.1699-0463.1953.tb00242.x). [PMID 13050415](https://pubmed.ncbi.nlm.nih.gov/13050415)

1. [*L.S.A., List of C.F.R. Sections Affected*](https://books.google.com/books?id=OLd91TkawxMC&pg=PA827). 2003. p. 827.

1. ["John Bellows Alsever"](https://medical-dictionary.thefreedictionary.com/John+Bellows+Alsever). *TheFreeDictionary.com*

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