# ASAP2

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**Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2** is a [protein](/source/Protein) that in humans is encoded by the *ASAP2* [gene](/source/Gene).[1][2][3]

This gene encodes a multidomain protein containing an [N-terminal](/source/N-terminal) [alpha-helical](/source/Alpha-helical) region with a [coiled-coil motif](/source/Coiled-coil_motif), followed by a [pleckstrin homology (PH) domain](/source/Pleckstrin_homology_domain), an Arf-GAP domain, an [ankyrin homology region](/source/Ankyrin_repeat), a [proline](/source/Proline)-rich region, and a [C-terminal](/source/C-terminal) [Src homology 3 (SH3) domain](/source/SH3_domain). The protein localizes in the [Golgi apparatus](/source/Golgi_apparatus) and at the [plasma membrane](/source/Plasma_membrane), where it colocalizes with [protein tyrosine kinase 2-beta](/source/Protein_tyrosine_kinase_2-beta) (PTK2B). The encoded protein forms a stable complex with PTK2B in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal [proline-rich domain](/source/Proline-rich_domain) of PTK2B. The encoded protein is [tyrosine phosphorylated](/source/Tyrosine_phosphorylation) by activated PTK2B. In vitro it shows strong [GTPase-activating protein](/source/GTPase-activating_protein) (GAP) activity towards the [small GTPases](/source/Small_GTPase) [ADP-ribosylation factor (ARF) 1](/source/ARF1) and [ARF5](/source/ARF5) and weak activity towards [ARF6](/source/ARF6). The encoded protein is believed to function as an ARF GAP that controls ARF-mediated [vesicle budding](/source/Vesicle_budding) when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PTK2B and [SRC](/source/Proto-oncogene_tyrosine-protein_kinase_Src), a pathway that may be involved in the regulation of vesicular transport.[3]

## Interactions

ASAP2 has been shown to [interact](/source/Protein-protein_interaction) with [PTK2B](/source/PTK2B).[4]

## References

1. Andreev J, Simon JP, Sabatini DD, Kam J, Plowman G, Randazzo PA, Schlessinger J (Mar 1999). "Identification of a New Pyk2 Target Protein with Arf-GAP Activity". *Mol Cell Biol*. **19** (3): 2338–50. [doi:10.1128/MCB.19.3.2338](https://doi.org/10.1128/MCB.19.3.2338). [PMC 84026](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84026). [PMID 10022920](https://pubmed.ncbi.nlm.nih.gov/10022920)

1. Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Feb 1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". *DNA Res*. **4** (5): 307–13. [doi:10.1093/dnares/4.5.307](https://doi.org/10.1093/dnares/4.5.307). [PMID 9455477](https://pubmed.ncbi.nlm.nih.gov/9455477)

1. ["Entrez Gene: DDEF2 development and differentiation enhancing factor 2"](https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=ShowDetailView&TermToSearch=8853)

1. Andreev, J; Simon J P; Sabatini D D; Kam J; Plowman G; Randazzo P A; Schlessinger J (Mar 1999). "Identification of a New Pyk2 Target Protein with Arf-GAP Activity". *Mol. Cell. Biol.*. **19** (3): 2338–50. [doi:10.1128/MCB.19.3.2338](https://doi.org/10.1128/MCB.19.3.2338). [ISSN 0270-7306](https://www.worldcat.org/issn/0270-7306). [PMC 84026](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC84026). [PMID 10022920](https://pubmed.ncbi.nlm.nih.gov/10022920)

## Further reading

- Brown MT, Andrade J, Radhakrishna H (1998). "ASAP1, a Phospholipid-Dependent Arf GTPase-Activating Protein That Associates with and Is Phosphorylated by Src". *Mol. Cell. Biol.*. **18** (12): 7038–51. [doi:10.1128/MCB.18.12.7038](https://doi.org/10.1128/MCB.18.12.7038). [PMC 109286](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC109286). [PMID 9819391](https://pubmed.ncbi.nlm.nih.gov/9819391)
- Kondo A, Hashimoto S, Yano H (2000). "A New Paxillin-binding Protein, PAG3/Papα/KIAA0400, Bearing an ADP-Ribosylation Factor GTPase-activating Protein Activity, Is Involved in Paxillin Recruitment to Focal Adhesions and Cell Migration". *Mol. Biol. Cell*. **11** (4): 1315–27. [doi:10.1091/mbc.11.4.1315](https://doi.org/10.1091/mbc.11.4.1315). [PMC 14849](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC14849). [PMID 10749932](https://pubmed.ncbi.nlm.nih.gov/10749932)
- Uchida H, Kondo A, Yoshimura Y (2001). "Pag3/Papα/Kiaa0400, a Gtpase-Activating Protein for Adp-Ribosylation Factor (Arf), Regulates Arf6 in Fcγ Receptor–Mediated Phagocytosis of Macrophages". *J. Exp. Med.*. **193** (8): 955–66. [doi:10.1084/jem.193.8.955](https://doi.org/10.1084/jem.193.8.955). [PMC 2193405](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193405). [PMID 11304556](https://pubmed.ncbi.nlm.nih.gov/11304556)
- Kim H, Lee Y (2002). "Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I". *Biochem. Biophys. Res. Commun.*. **289** (2): 513–8. [doi:10.1006/bbrc.2001.5992](https://doi.org/10.1006/bbrc.2001.5992). [PMID 11716503](https://pubmed.ncbi.nlm.nih.gov/11716503)
- Oshiro T, Koyama S, Sugiyama S (2002). "Interaction of POB1, a downstream molecule of small G protein Ral, with PAG2, a paxillin-binding protein, is involved in cell migration". *J. Biol. Chem.*. **277** (41): 38618–26. [doi:10.1074/jbc.M203453200](https://doi.org/10.1074/jbc.M203453200). [PMID 12149250](https://pubmed.ncbi.nlm.nih.gov/12149250)
- Strausberg RL, Feingold EA, Grouse LH (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". *Proc. Natl. Acad. Sci. U.S.A.*. **99** (26): 16899–903. [Bibcode:2002PNAS...9916899M](https://ui.adsabs.harvard.edu/abs/2002PNAS...9916899M). [doi:10.1073/pnas.242603899](https://doi.org/10.1073/pnas.242603899). [PMC 139241](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC139241). [PMID 12477932](https://pubmed.ncbi.nlm.nih.gov/12477932)
- Ota T, Suzuki Y, Nishikawa T (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". *Nat. Genet.*. **36** (1): 40–5. [doi:10.1038/ng1285](https://doi.org/10.1038/ng1285). [PMID 14702039](https://pubmed.ncbi.nlm.nih.gov/14702039)
- Hashimoto S, Hashimoto A, Yamada A (2004). "A novel mode of action of an ArfGAP, AMAP2/PAG3/Papalpha, in Arf6 function". *J. Biol. Chem.*. **279** (36): 37677–84. [doi:10.1074/jbc.M404196200](https://doi.org/10.1074/jbc.M404196200). [PMID 15231847](https://pubmed.ncbi.nlm.nih.gov/15231847)
- Kojima C, Hashimoto A, Yabuta I (2005). "Regulation of Bin1 SH3 domain binding by phosphoinositides". *EMBO J.*. **23** (22): 4413–22. [doi:10.1038/sj.emboj.7600442](https://doi.org/10.1038/sj.emboj.7600442). [PMC 526460](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC526460). [PMID 15483625](https://pubmed.ncbi.nlm.nih.gov/15483625)
- Gerhard DS, Wagner L, Feingold EA (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". *Genome Res.*. **14** (10B): 2121–7. [doi:10.1101/gr.2596504](https://doi.org/10.1101/gr.2596504). [PMC 528928](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC528928). [PMID 15489334](https://pubmed.ncbi.nlm.nih.gov/15489334)
- Onodera Y, Hashimoto S, Hashimoto A (2005). "Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities". *EMBO J.*. **24** (5): 963–73. [doi:10.1038/sj.emboj.7600588](https://doi.org/10.1038/sj.emboj.7600588). [PMC 554134](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC554134). [PMID 15719014](https://pubmed.ncbi.nlm.nih.gov/15719014)
- Olsen JV, Blagoev B, Gnad F (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". *Cell*. **127** (3): 635–48. [doi:10.1016/j.cell.2006.09.026](https://doi.org/10.1016/j.cell.2006.09.026). [PMID 17081983](https://pubmed.ncbi.nlm.nih.gov/17081983). [S2CID 7827573](https://api.semanticscholar.org/CorpusID:7827573)
- Ewing RM, Chu P, Elisma F (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". *Mol. Syst. Biol.*. **3** (1): 89. [doi:10.1038/msb4100134](https://doi.org/10.1038/msb4100134). [PMC 1847948](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847948). [PMID 17353931](https://pubmed.ncbi.nlm.nih.gov/17353931)

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Adapted from the Wikipedia article [ASAP2](https://en.wikipedia.org/wiki/ASAP2) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/ASAP2?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.