# ADA (buffer)

> Mediated Wiki article. Canonical URL: https://mediated.wiki/source/ADA_(buffer)
> Markdown URL: https://mediated.wiki/source/ADA_(buffer).md
> Source: https://en.wikipedia.org/wiki/ADA_(buffer)
> Source revision: 1294239300
> License: Creative Commons Attribution-ShareAlike 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/)

{{refimprove|date=November 2024}}
{{chembox
| Name = ADA
| ImageFile = ADA (buffer).png
| ImageName = Chemical structure of ADA
| SystematicName = 2,2′-[(2-amino-2-oxoethyl)azanediyl]diacetic acid
| OtherNames = ADA, N-(2-acetamido)iminodiacetic acid,  N-(carbamoylmethyl)iminodiacetic acid
|Section1={{Chembox Identifiers
| InChI = 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
| InChIKey = QZTKDVCDBIDYMD-UHFFFAOYSA-N
| SMILES = C(C(=O)N)N(CC(=O)O)CC(=O)O
| CASNo = 26239-55-4
| ChEBI = 43960
| DrugBank = DB02810 
| ChemSpiderID = 105243
| UNII = 5C4R3O704E
| EC_number = 247-530-0
| PubChem = 117765
  }}
| Section2 = {{Chembox Properties
| C=6 | H=10 | N=2 | O=5
| MolarMass = 190.155 g/mol
| pKa = 6.6
  }}
| Section3 = {{Chembox Hazards
| MainHazards = Irritant
| FlashPt = Non-flammable
| GHSSignalWord = Warning
| HPhrases = {{H-phrases|302|315|319|335}}
| PPhrases = {{P-phrases|261|264|270|271|280|301+312|302+352|304+340|305+351+338|312|321|330|332+313|337+313|362|403+233|405|501}}
  }}
}}

'''ADA''' is a [zwitterionic](/source/zwitterionic) organic chemical [buffering agent](/source/buffering_agent); one of [Good's buffers](/source/Good's_buffers). It has a useful [pH](/source/pH) range of 6.0-7.2 in the physiological range, making it useful for [cell culture](/source/cell_culture) work. It has a [pKa](/source/pKa) of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M [NaOH](/source/NaOH) where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a [chelating agent](/source/chelating_agent) for H<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup>, and for [isoelectric focusing](/source/isoelectric_focusing) in immobilized pH gradients. Its effects on dog kidney [Na<sup>+</sup>/K<sup>+</sup>-ATPase](/source/Na%2B%2FK%2B-ATPase) and rat brain [GABA receptor](/source/GABA_receptor)s have also been studied. ADA does, however, alter coloring in [bicinchoninic acid assay](/source/bicinchoninic_acid_assay)s.<ref>{{cite web| title=ADA| website=Sigma-Aldrich| url=http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/a9883pis.pdf}}</ref>

==References==
{{reflist}}

Category:Zwitterions
Category:Tertiary amines
Category:Dicarboxylic acids
Category:Buffer solutions

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Adapted from the Wikipedia article [ADA (buffer)](https://en.wikipedia.org/wiki/ADA_(buffer)) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/ADA_(buffer)?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.