# ACES (buffer)

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> Markdown URL: https://mediated.wiki/source/ACES_(buffer).md
> Source: https://en.wikipedia.org/wiki/ACES_(buffer)
> Source revision: 1174604826
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{{chembox
| Verifiedfields = changed
| Watchedfields = changed
| verifiedrevid = 477235210
| ImageFile=ACES_buffer.svg
| ImageSize=
| Name = ACES
| PIN=2-[(2-Amino-2-oxoethyl)amino]ethane-1-sulfonic acid
| OtherNames=''N''-(2-Acetamido)-2-aminoethanesulfonic acid
|Section1={{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID = 73843
| InChI = 1/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10)
| InChIKey = DBXNUXBLKRLWFA-UHFFFAOYAH
| SMILES1 = O=S(=O)(O)CCNCC(=O)N
| StdInChI_Ref = {{stdinchicite|correct|chemspider}}
| StdInChI = 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10)
| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}
| StdInChIKey = DBXNUXBLKRLWFA-UHFFFAOYSA-N
| CASNo_Ref = {{cascite|correct|CAS}}
| CASNo=7365-82-4
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = I5SPL5YVBY
| PubChem=81832
| ChEBI_Ref = {{ebicite|correct|EBI}}
| ChEBI = 39062
| SMILES=C(CS(=O)(=O)O)NCC(=O)N
  }}
|Section2={{Chembox Properties
| Formula =
| C=4 | H=10 | N=2 | O=4 | S=1 
| MolarMass=182.199
| Appearance=
| Density=
| MeltingPt=
| BoilingPt=
| Solubility=
  }}
|Section3={{Chembox Hazards
| MainHazards=
| FlashPt=
| AutoignitionPt =
  }}
}}

'''ACES''' ('''''N''-(2-acetamido)-2-aminoethanesulfonic acid''') is a chemical compound that is one of [Good's buffers](/source/Good's_buffers).  It was developed in the 1960s to provide buffer solutions with [pH](/source/pH) ranging from 6.15-8.35 for use in various applications.  With a [p''K''<sub>a</sub>](/source/pKa) of 6.9, it is often used as a [buffering agent](/source/buffering_agent) in [biological](/source/biology) and [biochemical](/source/biochemistry) research.  It is a [zwitterion](/source/zwitterion)ic buffer with a useful buffering range of 6.1-7.5.  The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.<ref>Good, N.E., "Hydrogen ion buffers for biological research." "Biochemistry", 5(2), 467–477.</ref>

==Applications==
ACES had been used to develop buffers for both [agarose](/source/agarose_gel_electrophoresis) and [polyacrylamide gel electrophoresis](/source/polyacrylamide_gel_electrophoresis).<ref>Liu, Q., et al., "pK-matched running buffers for gel electrophoresis." "Anal. Biochemistry.", 270(1):112-122.</ref>  
ACES use in [isoelectric focusing](/source/isoelectric_focusing) of [proteins](/source/proteins) has also been documented.<ref>Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." "Electrophoresis", 9(2):65-73.</ref>  Use of ACES has been published in a protocol for the analysis of bacterial [autolysins](/source/autolysins) in a discontinuous [SDS-PAGE](/source/SDS-PAGE) system.<ref>Strating, H., and Clarke, A.J., "Differentiation of bacterial autolysins by zymogram analysis." "Anal. Biochem.", 291(1):149-154.</ref>  Potential inhibition of ACES and other [Good buffers](/source/Good_buffers) has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.<ref>Tunnicliff, G., and Smith, J.A., "Competitive inhibition of γ-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-ε-ethanesulfonic acid and related buffer." "J. Neurochem.", 36(3):1122-1126.</ref>

==References==
<references/>

Category:Buffer solutions

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Adapted from the Wikipedia article [ACES (buffer)](https://en.wikipedia.org/wiki/ACES_(buffer)) by Wikipedia contributors ([contributor history](https://en.wikipedia.org/wiki/ACES_(buffer)?action=history)). Available under [Creative Commons Attribution-ShareAlike 4.0 International](https://creativecommons.org/licenses/by-sa/4.0/). Changes may have been made.